-
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTMP
Plasmid#129560Purposecytoplasmic markerDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQP-2363
Plasmid#138975PurposeRelocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illuminationDepositorInsertNcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_007_026_MV1
Plasmid#134419PurposepYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_008_026_MV1
Plasmid#134420PurposepYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pRPL18BAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_009_026_MV1
Plasmid#134421PurposepYTKMV1-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_007_026_MV2
Plasmid#134424PurposepYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_007_026_096
Plasmid#134410PurposepYTK096-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pTDH3Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_008_026_096
Plasmid#134411PurposepYTK096-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pRPL18BAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_009_026_096
Plasmid#134412PurposepYTK096-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_004_026_096
Plasmid#134407PurposepYTK096-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pTDH3Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_002_026_MV1
Plasmid#134414PurposepYTKMV1-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pRPL18BAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_006_026_096
Plasmid#134409PurposepYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pREV1Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_006_026_096
Plasmid#134418PurposepYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_004_026_MV2
Plasmid#134423PurposepYTKMV2-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_003_026_096
Plasmid#134406PurposepYTK096-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pREV1Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_005_026_MV1
Plasmid#134417PurposepYTKMV1-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pRPL18BAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_003_026_MV1
Plasmid#134415PurposepYTKMV1-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_001_026_MV1
Plasmid#134413PurposepYTKMV1-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_005_026_096
Plasmid#134408PurposepYTK096-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pRPL18BAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP
Plasmid#99730Purposeoverexpression of Tmeff2 and mCherryDepositorInsertTmeff2 (Tmeff2 Mouse)
UseTags2xMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
jk63
Plasmid#87011Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsCLIP-Nrx1B
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
jk68
Plasmid#87020Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-4xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
jk67
Plasmid#87013Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsCLIP-Nrx1B-4xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP430
Plasmid#87015Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsCLIP-Nrx1B-tdMS2(c)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP428
Plasmid#87014Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsCLIP-Nrx1B-1xλN(c)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
SUN2delCC
Plasmid#130836PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseTagsmCherry-FLAG-HAExpressionPlantMutationDeletion of coiled coil domainPromoterCaMV 35SAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Mouse Cherry Brie Pooled Library
Pooled Library#170511PurposeCRISPR library targeting mouse genesDepositorExpressionMammalianSpeciesSyntheticUseCRISPR and LentiviralAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMA-Cherry
Plasmid#110209PurposeMapping of mesenchymal progenitor cells.DepositorInsertmCherry
UseTagsNoneExpressionMammalianMutationPromoterSmooth muscle alpha-actin and first intronAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
actb2-SecAnxaV-mKate2-acry-EGFP
Plasmid#123628PurposeUbiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.DepositorInsertAnnexinA5
UseTagsmKate2ExpressionMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgLenti
Plasmid#105996Purposesingle sgRNA expression vectorDepositorTypeEmpty backboneUseCRISPR and Lentiviral; S.pyogenes sgrna expressio…TagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLIM-AKAR
Plasmid#63058PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backboneDepositorInsertFLIM-AKAR
UseAAVTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-Blast
Plasmid#159741PurposeExpresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-1xStop
Plasmid#164411PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY1243-Fluorescent RADARS
Plasmid#182540PurposeExpress Fluorescent RADARS in bicistronic vector with mCherry as control geneDepositorInsertmNeon
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-mito-EGFP
Plasmid#159096PurposeExpress EGFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitochondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMN-332
Plasmid#195466PurposeLentiviral Integratable Inducible synZiFTR ZF1 + ReporterDepositorInsertinverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hRIP3 dRHIM
Plasmid#208788PurposeInducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2DepositorInsertRIP3 (RIPK3 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationRHIM domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAMA-empty
Plasmid#74755PurposeMammalian lentiviral vectorDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX
Plasmid#188994PurposeA plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…PromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianMutationPromoterLac promoter, SV40, and T7 promoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-RhoA
Plasmid#42958PurposeExpresses optogenetically controlled RhoADepositorInsertCry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-NBL10
Plasmid#69971PurposeCre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusionDepositorInsertNBL10
UseAAV and Cre/LoxTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL0341 (pTarget)
Plasmid#200906PurposeEncodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.DepositorInsertpTarget
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only