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pLCK-mScarlet3_C1

Plasmid

#189771

Purpose

Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LCK-mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDae079 (eMutaT7[transition])

Plasmid

#187622

Purpose

Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Publication

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Inserts

TadA-8e-T7RNAP
PmCDA1-T7RNAP

Expression

Bacterial

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Publication

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Flag-FOXM1

Plasmid

#153136

Purpose

Expression of Flag tagged human FOXM1 in mammalian cells

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXM1 (FOXM1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

Plasmid

#118154

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

EF1a core and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti-ABERA-P2A-Puro

Plasmid

#112675

Purpose

Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

ABERA

Use

Lentiviral

Tags

FLAG

Mutation

D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Publication

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Promoter

None

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pmScarlet_peroxisome_C1

Plasmid

#85063

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEIGW roGFP2-Orp1

Plasmid

#64993

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Str-KDEL_TNF-SBP-EGFP

Plasmid

#65278

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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hTLR3-pcDNA3

Plasmid

#32712

Depositor

Saumen Sarkar

Publication

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

TLR3 (TLR3 Human)

Tags

FLAG and Preprototrypsin

Expression

Mammalian

Promoter

CMV

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMV306hsp+Lux

Plasmid

#26159

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p4458 FLAG-hPLIC-1

Plasmid

#8663

Depositor

Peter Howley

Publication

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-1 (UBQLN1 Human)

Tags

FLAG

Expression

Mammalian

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles
RCAS-K-ras

Plasmid

#11549

Depositor

Sandra Orsulic

Publication

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)

Use

Retroviral

Mutation

G12D

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pT7-VP3SA11

Plasmid

#89164

Purpose

Express Rotavirus SA11 VP3 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP3

Expression

Mammalian

Promoter

T7 promotor

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMXs-Ms-L-Myc

Plasmid

#26023

Depositor

Shinya Yamanaka

Publication

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

L-Myc (Mycl Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-HA-hIFITM1

Plasmid

#58399

Purpose

Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM1 (IFITM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG423GAL-ccdB

Plasmid

#14149

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pCMV-PE6a

Plasmid

#207851

Purpose

Mammalian expression of PE6a prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6a

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPSU150

Plasmid

#173836

Purpose

Expresses 150 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABMBPpepN

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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