We narrowed to 14,661 results for: Lif

1. α-synuclein-3H

   Plasmid

   #205715

   Purpose

   Mammalian expression of alpha-synuclein-3H

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   thermostable three helix-bundle protein 3H

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-αS-2

   Plasmid

   #205706

   Purpose

   Mammalian expression of alpha-synuclein tandem dimer

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   alpha-synuclein tandem dimer (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM11-6xHis-TEV-hORF1p_StammerAAA

   Plasmid

   #202587

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-80L 2xHis-Rab12 Q101L

   Plasmid

   #207347

   Purpose

   Bacterial expression of Rab12

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   Rab12 (RAB12 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   Q101L

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Best1-N.Txnip

   Plasmid

   #206348

   Purpose

   AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N terminal half (1-228aa)

   Promoter

   human Best1

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Best1-sC.Txnip

   Plasmid

   #206351

   Purpose

   AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Short C terminal half (255-397aa)

   Promoter

   human Best1

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Best1-nt.Txnip(C247S)(1-320aa)

   Plasmid

   #206352

   Purpose

   AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   (1-320aa), C247S

   Promoter

   human Best1

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-RO1.7-dnHIF

   Plasmid

   #206343

   Purpose

   AAV plasmid expressing dominant negative HIF1A in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

   Insert

   dominant negative hif1a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   dominant negative HIF1A

   Promoter

   1.7kb human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-RedO-nt.Txnip(C247S)(1-301aa)

   Plasmid

   #206354

   Purpose

   AAV plasmid expressing deltion and mutant TXNIP in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   (1-301aa), C247S

   Promoter

   human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-EGFPnls barcode-210-SV40 polyA

    Plasmid

    #190876

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode210
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-EGFP barcode-9-SV40 polyA

    Plasmid

    #190873

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFsynW SYT1deltaC2AB reporter

    Plasmid

    #197279

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pminiTol2-4kb wnt3 promoter-PMTmApple

    Plasmid

    #203794

    Purpose

    Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesis

    Depositor

    Thorsten Wohland

    Article

    Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

    Insert

    PMT
    

    Use

    Zebrafish expression

    Tags

    mApple

    Expression

    Mutation

    Promoter

    4kb Wnt3 promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-THTAP-SPARTIN, residues 1-101

    Plasmid

    #180626

    Purpose

    Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPARTIN
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-101

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-THTAP-CALPAIN-7 1-165

    Plasmid

    #180610

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-65

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA528 CALPAIN-7 residues 1-165

    Plasmid

    #180611

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TBPL1 KO 1

    Plasmid

    #200210

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 1 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TBPL1 KO 2

    Plasmid

    #200211

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 2 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-guide-puro mYwhae -2

    Plasmid

    #198498

    Purpose

    lentiviral stable expression of mYwhae gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-guide-puro mYwhae - 1

    Plasmid

    #198497

    Purpose

    lentiviral stable expression of mYwhae gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge