We narrowed to 3,492 results for: aaas

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pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106355

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

MYOD1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.CMV/CB-EGFP

Plasmid

#121508

Purpose

Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfTuba_1

Plasmid

#26717

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Tuba RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pORANGE Cacnb4-GFP KI

Plasmid

#139663

Purpose

Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_BCL6_iso1_2

Plasmid

#104047

Purpose

Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

BCL6_iso1 gRNA (BCL6 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

Plasmid

#178110

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfTuba_2

Plasmid

#26718

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Tuba_2 RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
AURKA A2.1 gRNA

Plasmid

#90541

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKA (Guide Designation A2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pOPTXcGMPRELUC

Plasmid

#68503

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)

Tags

luciferase

Expression

Bacterial and Plant

Mutation

3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

Promoter

OPTX promoter with cGMPRE

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
UBA1 G5.3 gRNA

Plasmid

#90932

Purpose

3rd generation lentiviral gRNA plasmid targeting human UBA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UBA1 (Guide Designation G5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_sgGFP3

Plasmid

#119875

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSuper-PACSIN1-shRNA

Plasmid

#72576

Purpose

Expresses shRNA against PACSIN1

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

PACSIN1 (Pacsin1 Mouse)

Use

RNAi

Expression

Mammalian

Promoter

H1 promoter

Available Since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

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PRC1 G2.2 gRNA

Plasmid

#90857

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PRC1 (Guide Designation G2.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo U6-SpAi9L-U6SpDMDL

Plasmid

#78608

Purpose

Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9L-U6SpDMDL

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
pBW1997_pExpr-hU6-CRISPR-DECODER

Plasmid

#87549

Purpose

Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

gRNAs targeting NGN2, MIAT, ACTC1, TTN

Use

CRISPR, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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EFNB2-gRNA3/4

Plasmid

#139973

Purpose

gRNA used for knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EFNB2-gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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CSNK1A1 H2.4 gRNA

Plasmid

#90641

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CSNK1A1 (Guide Designation H2.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh1

Plasmid

#41927

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pPICZ:hTRAAK(G124I)

Plasmid

#130672

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, G124I

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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FBXO5 D3.4 gRNA

Plasmid

#90686

Purpose

3rd generation lentiviral gRNA plasmid targeting human FBXO5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FBXO5 (Guide Designation D3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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