We narrowed to 5,851 results for: KRAS

Showing: 2401 - 2420 of 5851 results

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eNme2-C TadCBEa

Plasmid

#193840

Purpose

Express TadCBEa (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pProEx Rab1A(S20C) CL

Plasmid

#224272

Purpose

Protein purification of Rab1A (S20C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rab1A (RAB1A Human)

Expression

Bacterial

Mutation

S20C

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAT15415-BEAR-GFP-target-mCherry

Plasmid

#162995

Purpose

plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-GFP plasmid

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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AA025

Plasmid

#216005

Purpose

Fragmid fragment: (Cas protein) NG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-NG_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA299

Plasmid

#216029

Purpose

Fragmid fragment: (Cas protein) NNGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Slug]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA296

Plasmid

#216028

Purpose

Fragmid fragment: (Cas protein) NNNNCC PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Nme2Cas9_v1.1 [Nme2]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA180

Plasmid

#216023

Purpose

Fragmid fragment: (Cas protein) NGRR(T) PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Sa]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA301

Plasmid

#216030

Purpose

Fragmid fragment: (Cas protein) NNGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Sauri]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA027

Plasmid

#216007

Purpose

Fragmid fragment: (Cas protein) nearly PAM-less

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-SpRY_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA026

Plasmid

#216006

Purpose

Fragmid fragment: (Cas protein) NG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-SpG_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pIN4_tsp-6_mScarlet

Plasmid

#182935

Purpose

Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Promoter

tsp-6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Arch(D95H)-eGFP

Plasmid

#51081

Purpose

Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

Depositor

Adam Cohen

Article

Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:

Insert

Archaerhodopsin-3 w/ D95H point mutation fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Histidine

Promoter

Ubiquitin

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pINDgw RFA-V5-EcoDam

Plasmid

#59204

Purpose

Mammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Tags

6xHA

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V2

Plasmid

#19968

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)

Tags

C terminal Venus and Flag

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
Axin1_HA in pRK5

Plasmid

#154220

Purpose

C-terminal HA-tagged AXIN1 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

AXIN1 (AXIN1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGMEH-T38M-750-luxAB-DAS+4

Plasmid

#49520

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

T38M-P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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