We narrowed to 12,169 results for: Ski

1. NLS-tdPCP-GFP-VPR

   Plasmid

   #183936

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-GFP-VPR
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHP138 Myc-TRF2-deltaB deltaM

   Plasmid

   #164255

   Purpose

   for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TRF2
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   deletion of amino acids-86 and 496-542

   Promoter

   Tetracycline inducible promotor

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 shSV40 neo

   Plasmid

   #158646

   Purpose

   Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   SV40Tag (SV40gp6 Simian virus 40)
   

   Use

   Lentiviral

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pscALPS- HIV2-ROD vpx

   Plasmid

   #115816

   Purpose

   Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HIV2ROD Vpx (M15390)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Promoter

   SFFV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 CIRBP

   Plasmid

   #67055

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CIRBP (CIRBP Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. NLS-dCas9-HA-NLS-GFP-VP16

   Plasmid

   #183923

   Purpose

   VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-VP16
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS PKC theta

   Plasmid

   #8426

   Depositor

   Frederic Mushinski

   Article

   Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

   Insert

   Mouse Protein Kinase C theta (Prkcq Mouse)
   

   Expression

   Bacterial

   Available Since

   Nov. 22, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pLX302 OAS2-V5 puro

   Plasmid

   #158642

   Purpose

   Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   OAS2 (OAS2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 IL6

   Plasmid

   #67061

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL6 (IL6 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pQCXIZ-turq-3xFLAG-TREX1 D18N

    Plasmid

    #164233

    Purpose

    retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and mturquoise

    Expression

    Mammalian

    Mutation

    D18N ->nuclease deficient

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJEP300-pAAV-CMV-MCS2-pA

    Plasmid

    #112138

    Purpose

    CMV driven Multi Cloning Site-2

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3_5UTR_Myc_FLuc

    Plasmid

    #229502

    Purpose

    Myc 5' untranslated region firefly luciferase reporter.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    Myc 5'UTR
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pscALPS-HIV-1 Group P-vpr

    Plasmid

    #115837

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group P Vpr (HQ179987)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. RhoC FLARE.sc mCer, mVenus - wt

    Plasmid

    #65071

    Purpose

    RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus

    Promoter

    CMV, p10

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pFA6a-link-GFPEnvy-SpHis5

    Plasmid

    #60782

    Purpose

    Yeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selection

    Depositor

    Linda Huang

    Article

    Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

    Insert

    GFPEnvy
    

    Use

    Yeast integrating

    Mutation

    S30R Y39N T65C S72A F99S N105T Y145F M153T V163A …

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTH PKC alpha

    Plasmid

    #8409

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C alpha (Prkca Mouse)
    

    Expression

    Mammalian

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscALPS gag-gfp/vpx

    Plasmid

    #115808

    Purpose

    SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-CRY2-mCh-superPLDx10

    Plasmid

    #188986

    Purpose

    A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CRY2 and mCherry

    Expression

    Mammalian

    Mutation

    F163L, K249R, G328S, G381V, G429D, D480G

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-D4 miR30-SUV39h1 ts2

    Plasmid

    #115881

    Purpose

    SUV39h1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h1 shRNA (SUV39H1 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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