We narrowed to 23,621 results for: crispr

1. pNW3

   Plasmid

   #53372

   Purpose

   Csy4 and Cas9 D10A nickase expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9n (D10A)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRU206

   Plasmid

   #167693

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUBQ4-2::asCpf1
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRU322

   Plasmid

   #167683

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::asCas9
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRU293

   Plasmid

   #167688

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMVP-Cas9C

   Plasmid

   #80939

   Purpose

   Plasmid that expresses Cas9C in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBO.ACT003

   Plasmid

   #182713

   Purpose

   aTc inducible dCasRx-IF3

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   3x(GGGS)-Escherichia Coli Initiation Factor 3

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pTet

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCCM935

   Plasmid

   #58202

   Purpose

   Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

   Depositor

   Craig Mello

   Article

   Kim et al Genetics. 2014 May 30. pii: genetics.114

   Insert

   unc-22 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

   Plasmid

   #170182

   Purpose

   Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   mCherry
   

   Tags

   3XFlag and T2A

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pADH119

   Plasmid

   #90985

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFC331

    Plasmid

    #87843

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-T3-hCasD10A-pA

    Plasmid

    #51638

    Purpose

    Expresses Cas9-nickase in mammalian cells and zygotes.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

    Insert

    Codon-optimized Cas9 D10A
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pFH6_new

    Plasmid

    #105866

    Purpose

    more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

    Depositor

    Andreas Weber

    Article

    Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Subcloning

    Expression

    Plant

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRE57I-GNAc

    Plasmid

    #170637

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, GNAc cassette, lacI
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBY2_ligD(FnCpf1)

    Plasmid

    #104623

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    crRNA to ligD
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pj23119

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pColE1_sgRNA_M1S_AmpR

    Plasmid

    #119153

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColE1_sgRNA_T2_AmpR

    Plasmid

    #119159

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pColE1_sgRNA_T3_AmpR

    Plasmid

    #119160

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pColE1_sgRNA_T1_AmpR

    Plasmid

    #119158

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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