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PCMV-intron myc Rab6 T27N

Plasmid

#46782

Purpose

expression of T27N Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 T27N (RAB6A Human)

Tags

myc

Expression

Mammalian

Mutation

T27N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB

Plasmid

#216233

Purpose

S. aureus USA300 GyrB Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_UCP2

Plasmid

#132286

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP2 (UCP2 Human)

Expression

Mammalian

Available Since

Nov. 13, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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vDip-C: pAAV-Pcp2-mGreenLantern-KASH

Plasmid

#207613

Purpose

vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

Depositor

Longzhi Tan

Article

Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

Insert

mGreenLantern

Use

AAV

Tags

KASH

Promoter

mouse Pcp2 promoter (L7-6)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX3X-RanBP2dFG

Plasmid

#53120

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2553-2838)

Promoter

tac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pKLBac-Bat-FluA-trimer

Plasmid

#229858

Purpose

Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

Inserts

PA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:icre-p2a-tomato

Plasmid

#135217

Purpose

Cre coexpressed with tdTomato in macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter with iCre and Tomato

Use

Cre/Lox; Zebrafish expression

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK3-2A-GFP

Plasmid

#118286

Purpose

Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)

Use

AAV

Tags

2A peptide and GFP

Expression

Mammalian

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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