We narrowed to 7,591 results for: Lif;

1. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

   Plasmid

   #127355

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pES002 MBP-GSK3β_S9A-HA-His

   Plasmid

   #196184

   Purpose

   Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Tags

   HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

   Plasmid

   #185676

   Purpose

   SpCas9 and gRNA targeting the C-terminus of HsIRE1

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 gRNA (ERN1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

4. pBbA5a-sufCDSUB

   Plasmid

   #195055

   Purpose

   Expresses SUF operon from B. subtilis from IPTG-inducible promoter

   Depositor

   Greg Bokinsky

   Article

   D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

   Insert

   sufCDSUB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCB306-GFP-Rab8A

   Plasmid

   #198470

   Purpose

   lentiviral expression of GFP-Rab8A

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   GFP-Rab8A (RAB8A Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIA10.5-Clover

   Plasmid

   #214449

   Purpose

   BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   mClover
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTMiniTrex-mCherry-Shark2

   Plasmid

   #233144

   Purpose

   Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

   Depositor

   Ronald Etheridge

   Article

   Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

   Inserts

   mCherry
   Shark2
   

   Use

   Prokaryotic expression

   Tags

   3x Ty tag

   Expression

   Mutation

   Codon optimized for Trypanosoma cruzi

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGPN-TMPRSS2

   Plasmid

   #187484

   Purpose

   Expression vector - TMPRSS2

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTT3 Unc5C-FLAG

   Plasmid

   #72196

   Purpose

   Expresses full-length Unc5c with a C-terminal FLAG tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5c (Unc5c Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-PDGFRB-V5/HIS

    Plasmid

    #201988

    Purpose

    expression of human PDGFRB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRB receptor tyrosine kinase (PDGFRB Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC150-RFP-EGFP-LGALS3 R186S

    Plasmid

    #175779

    Purpose

    PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R186S

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mcherry p62 delta UBA silent

    Plasmid

    #187984

    Purpose

    mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacking UBA domain (aa389-434) but it has the sil…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG_Xph20-GCaMP7f

    Plasmid

    #135539

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    GCaMP7f

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mcherry-p62 delta PB1 silent

    Plasmid

    #187985

    Purpose

    human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacking PB1 domain (aa1-102) with a silent mutati…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid

    #127369

    Purpose

    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKrox24(MapErk)dTomato

    Plasmid

    #200113

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-TRKC-V5/HIS

    Plasmid

    #205615

    Purpose

    Expression of human TRKC receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TRKC receptor tyrosine kinase (NTRK3 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTT3 Unc5D-FLAG

    Plasmid

    #72197

    Purpose

    Expresses full-length Unc5d with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5d (Unc5d Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-RON-V5/HIS

    Plasmid

    #201983

    Purpose

    expression of human RON receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RON receptor tyrosine kinase (MST1R Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

    Plasmid

    #100846

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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