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We narrowed to 2,492 results for: PUC19

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Showing: 2421 - 2440 of 2492 results

  4. pZNF57-donor

    Plasmid
    #112296
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF57 with GFP
    Depositor
    Kevin White
    Insert
    ZNF57 homology arms flanking EGFP-IRES-Neo cassette (ZNF57 Human)
    Use
    CRISPR
    Mutation
    rs2288959, rs2288958, rs10410539, hg38:19:2918211…
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112296-plasmid-map-sequence-id-225584

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  5. pNR2F1-donor

    Plasmid
    #112388
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR2F1 with GFP
    Depositor
    Kevin White
    Insert
    NR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
    Use
    CRISPR
    Mutation
    rs974916569
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112388-plasmid-map-sequence-id-229852

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  6. pTBPL1-donor

    Plasmid
    #112316
    Purpose
    CRISPR donor plasmid to tag human transcription factor TBPL1 with GFP
    Depositor
    Kevin White
    Insert
    TBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
    Use
    CRISPR
    Mutation
    rs35776966, hg38:6:133987646:GTGTAT>-
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112316-plasmid-map-sequence-id-228480

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  7. pSMAD3-donor

    Plasmid
    #112311
    Purpose
    CRISPR donor plasmid to tag human transcription factor SMAD3 with GFP
    Depositor
    Kevin White
    Insert
    SMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
    Use
    CRISPR
    Mutation
    hg38:15:67189762:C>T, rs11629568, rs8025774
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112311-plasmid-map-sequence-id-228463

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  8. pYI2294

    Plasmid
    #228352
    Purpose
    Methanol-inducible ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    KpAOX1 promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228352-plasmid-map-sequence-id-457091

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  9. pYI2293

    Plasmid
    #228351
    Purpose
    Constitutive ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228351-plasmid-map-sequence-id-457078

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  10. qTAG-N-Blast-moxGFP-LMNB1

    Plasmid
    #207772
    Purpose
    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Feb. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    207772-plasmid-map-sequence-id-415638

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  11. qTAG-N-Blast-miniTurbo-LMNB1

    Plasmid
    #207774
    Purpose
    Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207774-plasmid-map-sequence-id-416197

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  13. pVEZF1-donor

    Plasmid
    #112341
    Purpose
    CRISPR donor plasmid to tag human transcription factor VEZF1 with GFP
    Depositor
    Kevin White
    Insert
    VEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
    Use
    CRISPR
    Mutation
    rs999907002, hg38:17:57975308:A>T, hg38:17:579…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112341-plasmid-map-sequence-id-229654

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  14. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

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  15. qTAG-C-Solo-mScarlet-H3C2

    Plasmid
    #207781
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207781-plasmid-map-sequence-id-416187

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  19. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

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  20. pYI2216

    Plasmid
    #228349
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228349-plasmid-map-sequence-id-457093

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Showing: 2421 - 2440 of 2492 results