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We narrowed to 13,416 results for: sequence

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Showing: 2421 - 2440 of 13416 results

  2. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid
    #177723
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177723-plasmid-map-sequence-id-352618

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  3. p406 – TDH3-ER-FlucDM-GFP11-Ura

    Plasmid
    #177731
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Tags
    GFP11, HA, HDEL, and KAR2 Signal Sequence
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177731-plasmid-map-sequence-id-352614

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  4. p406 – TDH3-ER-FlucDM-GFP- Ura

    Plasmid
    #177732
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Tags
    HA, HDEL, KAR2 Signal Sequence, and eGFP
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177732-plasmid-map-sequence-id-352622

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  6. CMV-10xMS2 no wild type

    Plasmid
    #158204
    Purpose
    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of MS2 without the WT sequence from oligo library experiment
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158204-plasmid-map-sequence-id-311816

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  7. CMV-10xQb no wild type

    Plasmid
    #158205
    Purpose
    Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158205-plasmid-map-sequence-id-311817

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  8. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126758
    Purpose
    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126758-plasmid-map-sequence-id-412365

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  9. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126759
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126759-plasmid-map-sequence-id-412375

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  11. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126754
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126754-plasmid-map-sequence-id-410972

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  12. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid
    #126755
    Purpose
    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126755-plasmid-map-sequence-id-410971

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  13. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126756
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126756-plasmid-map-sequence-id-410579

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  14. pET24a-VHH-tev-2xTS

    Plasmid
    #117750
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117750-plasmid-map-sequence-id-228351

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  16. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  17. SMR3B-bio-His

    Plasmid
    #52183
    Purpose
    Expresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SMR3B (SMR3B Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52183-plasmid-map-sequence-id-80946

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  18. CD84-bio-His

    Plasmid
    #51649
    Purpose
    Expresses full-length SLAM family member 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD84 (CD84 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51649-plasmid-map-sequence-id-78973

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  20. pAAV-CAG-Flex-Rev-3xGFP

    Plasmid
    #122500
    Purpose
    Cre-dependent GFP expression.
    Depositor
    Kimberly Ritola
    Insert
    3x GFP
    Use
    AAV
    Mutation
    each GFP has unique nt sequence
    Available Since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    122500-plasmid-map-sequence-id-237802

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Showing: 2421 - 2440 of 13416 results