We narrowed to 12,861 results for: sequence

1. EF1a_GATA4_P2A_Hygro_Barcode

   Plasmid

   #120444

   Purpose

   Barcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLOD2-bio-His

   Plasmid

   #51756

   Purpose

   Expresses full-length Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PLOD2 (PLOD2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJM656

   Plasmid

   #53086

   Purpose

   A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

   Depositor

   Ding Xue

   Article

   Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

   Insert

   Lactaherin (Mfge8 Mouse)
   

   Use

   Tags

   GFP (w/ introns) and signal sequence

   Expression

   Worm

   Mutation

   deleted native signal sequence, deleted C-termina…

   Promoter

   hsp-16.41

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCBP1_Full-length_pGEX

   Plasmid

   #135108

   Purpose

   Recombinant expression of PCBP1 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PCBP1 (PCBP1 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-His6-SUMO-cSrc-SH2-AviTag

   Plasmid

   #214230

   Purpose

   Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

   Insert

   cSrc kinase SH2 domain (SRC Human)
   

   Use

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_TFAP2C_P2A_Hygro_Barcode

   Plasmid

   #120487

   Purpose

   Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   TFAP2C (TFAP2C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmiCas9-msa

   Plasmid

   #164833

   Purpose

   miCas9-msa fusion protein expressing plasmid

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miCas9-msa
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   contains mSA sequence range is 5106-5447 and Brex…

   Promoter

   CMV

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-EphB3-Fc

   Plasmid

   #200985

   Purpose

   Mammalian expression plasmid for soluble EphB3 fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB3 (EPHB3 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Soluble ectodomain of EphB3

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_RUNX1_P2A_Hygro_Barcode

   Plasmid

   #120475

   Purpose

   Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   RUNX1 (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a_HAND2_P2A_Hygro_Barcode

    Plasmid

    #120447

    Purpose

    Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEAT8-137 M1V

    Plasmid

    #173807

    Purpose

    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

    Depositor

    Anne Gershenson

    Article

    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

    Insert

    SERPiNA1 (SERPINA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E1M Otherwise this plasmid encodes the most commo…

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NOTCH3-bio-His

    Plasmid

    #53343

    Purpose

    Expresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EF1a_FLI1_P2A_Hygro_Barcode

    Plasmid

    #120437

    Purpose

    Barcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EF1a_ERG_P2A_Hygro_Barcode

    Plasmid

    #120434

    Purpose

    Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ERG (ERG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMS-dsRed2-WT LMNA-GFP minigene

    Plasmid

    #128230

    Purpose

    Lamin A splicing reporter

    Depositor

    Tom Misteli

    Article

    Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

    Insert

    lamin A (LMNA Human)
    

    Use

    Tags

    GFP and SV40-driven dsRed2 to identify transfecte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IGF2BP1_2xRRM-4xKH_pGEX

    Plasmid

    #135122

    Purpose

    Recombinant expression of IGF2BP1 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBait-YY1

    Plasmid

    #165147

    Purpose

    Bait plasmid for PROBER with YY1-binding site (positive control)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    3X YY1 motif
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TGFB1-bio-His

    Plasmid

    #52185

    Purpose

    Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TGFB1 (TGFB1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ITIH4-bio-His

    Plasmid

    #52181

    Purpose

    Expresses full-length Inter-alpha-trypsin inhibitor heavy chain H4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ITIH4 (ITIH4 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge