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We narrowed to 9,529 results for: sgRNA

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Showing: 2421 - 2440 of 9529 results

  10. pSCBE3-NG-HF1-Hypa

    Plasmid
    #218162
    Purpose
    This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218162-plasmid-map-sequence-id-433200

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  11. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid
    #136660
    Purpose
    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136660-plasmid-map-sequence-id-273363

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  12. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126778
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R691A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126778-plasmid-map-sequence-id-259276

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  16. pFH99

    Plasmid
    #128204
    Purpose
    classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    Depositor
    Vladimir Nekrasov
    Publication
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128204-plasmid-map-sequence-id-252914

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  17. pFH100

    Plasmid
    #128205
    Purpose
    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    Depositor
    Vladimir Nekrasov
    Publication
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128205-plasmid-map-sequence-id-252913

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  18. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chick U6.3
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92394-plasmid-map-sequence-id-191866

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Showing: 2421 - 2440 of 9529 results