We narrowed to 3,068 results for: KIS

1. HR700_HA2TP53_Ex8

   Plasmid

   #228863

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pKM-U6-reRNA-hsRUNX

   Plasmid

   #205639

   Purpose

   reRNA guide targeting Homo sapiens RUNX

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-hsRUNX (RUNX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK189

   Plasmid

   #219743

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK4169

   Plasmid

   #219744

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304_miRFP670nano-P2A-ERM-gCam6f

   Plasmid

   #214927

   Purpose

   Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)

   Depositor

   Xinnan Wang

   Article

   Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi

   Insert

   miRFP670nano-P2A-ERM-gCam6f
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVp

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 puro shCALM2-1

   Plasmid

   #193699

   Purpose

   Constitutive lentiviral expression of CALM2 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   CALM2 (CALM2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 puro shCALM2-2

   Plasmid

   #193700

   Purpose

   Constitutive lentiviral expression of CALM2 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   CALM2 (CALM2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 puro shCALM2-3

   Plasmid

   #193701

   Purpose

   Constitutive lentiviral expression of CALM2 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   CALM2 (CALM2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCUU

   Plasmid

   #159747

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223 ATG3 C264A

    Plasmid

    #129293

    Purpose

    Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    changed Cysteine 264 to Alanine

    Promoter

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 ATG3 allKR

    Plasmid

    #129295

    Purpose

    Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    changed all 22 Lysine residues to Arginine

    Promoter

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV 3xFLAG-ATG3 allKR

    Plasmid

    #129298

    Purpose

    Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed all 22 Lysine residues to Arginine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV 3xFLAG-ATG3 1-13 KR

    Plasmid

    #129299

    Purpose

    Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed first 13 Lysine residues to Arginine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV 3xFLAG-ATG3 14-22 KR

    Plasmid

    #129300

    Purpose

    Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed last 9 Lysine residues to Arginine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV 3xFLAG-ATG3 K295R

    Plasmid

    #129302

    Purpose

    Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 295 to Arginine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5-LAP-MPS1-dTPR

    Plasmid

    #114048

    Purpose

    Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-dTPR (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta TPR (deletion of TPR domain 63-191)

    Promoter

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5-LAP-MPS1-d60

    Plasmid

    #114046

    Purpose

    Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-d60 (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta 60 (deletion of NTE domain)

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. px330-Axin1

    Plasmid

    #162543

    Purpose

    sgRNA targeting Axin1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Axin1 (Axin1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn-DIO-PinkyCaMP

    Plasmid

    #232857

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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