We narrowed to 18,992 results for: Met

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Parp14-c011

Plasmid

#173098

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K791-F978

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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rArc

Plasmid

#178206

Purpose

Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

Depositor

Stefan Heinemann

Article

Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

Insert

rArc

Expression

Mammalian

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB

Plasmid

#11777

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBL4

Plasmid

#50548

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pReporter_12

Plasmid

#60572

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_12

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSkipFlox

Plasmid

#85797

Purpose

P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

split Cre recombinase

Use

Cre/Lox; P. falciparum expression

Tags

FKBP, FRB*

Promoter

Pfcrt

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Gi-c010

Plasmid

#173080

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GAP

Tags

mCitrine

Expression

Bacterial

Mutation

mCitrine + last 10 residues of GNA1, codon optimi…

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxOFF-pre-mGRASP-mCerulean

Plasmid

#51900

Purpose

JxOFF-pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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VSVGwt-SBP-EGFP_puromycin

Plasmid

#65302

Purpose

VSVG reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_puromycin

Plasmid

#65309

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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ii-Str_SBP-mCherry-GPI

Plasmid

#65297

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJMK004

Plasmid

#34616

Purpose

Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator

Depositor

Adam Cohen

Article

Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n

Insert

Arch D95N

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Asparagine

Promoter

CaMKII

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pBCN27-Pmyo-2::GFP::unc-54_3'UTR

Plasmid

#26347

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Insert

Pmyo-2::GFP::unc-54_3'UTR

Expression

Worm

Available Since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pNB769

Plasmid

#60138

Purpose

Red click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Expression

Yeast

Promoter

MET17pr

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pRATIO4212

Plasmid

#141313

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Tags

GST

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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