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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,970 results for: T7

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Showing: 2441 - 2460 of 10970 results

  1. GLI2 his

    Plasmid
    #87097
    Purpose
    expresses GLI2 in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    GLI2 (GLI2 Human)
    Tags
    6x His, T7 tag, and Xpress tag
    Expression
    Mammalian
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    87097-plasmid-map-sequence-id-201477

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  11. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  13. pHIS-GB1-BP1

    Plasmid
    #133427
    Purpose
    Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    SREBF1 (SREBF1 Human)
    Expression
    Bacterial
    Mutation
    SREBP1a amino acids 17-42 fused to GB1 solubilit…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133427-plasmid-map-sequence-id-267726

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  15. MARS_pNIC-CTB10H

    Plasmid
    #153053
    Purpose
    Expression construct for producing Avi tagged Methionyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    MARS (MARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153053-plasmid-map-sequence-id-304471

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  16. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  17. His 3C MKK6 1-334

    Plasmid
    #87881
    Purpose
    E. coli expression of MKK6 aa 1-334
    Depositor
    Peter Mace
    Insert
    MKK6 (MAP2K6 Human)
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-334
    Promoter
    T7
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87881-plasmid-map-sequence-id-174845

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  18. TRMT10C (RG9MTD1A-c113)

    Plasmid
    #110248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RG9MTD1 (TRMT10C Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110248-plasmid-map-sequence-id-212286

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  19. KDM4D

    Plasmid
    #73247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KDM4D (KDM4D Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam02375:JmjN 18-64, pfam02373:JmjC 182-295
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73247-plasmid-map-sequence-id-137202

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  20. GFP-A96 ELP

    Plasmid
    #67856
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residue
    Depositor
    Andrew MacKay
    Insert
    GFP fused to ELP (VPGAG) repeated 96 times
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Promoter
    T7
    Available Since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67856-plasmid-map-sequence-id-117813

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Showing: 2441 - 2460 of 10970 results