We narrowed to 5,055 results for: cre encoding plasmid

1. TU#1806_CRISPR_unc-73_exon21

   Plasmid

   #82360

   Purpose

   to create unc-73E null allele

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTSAR3.7t

   Plasmid

   #210259

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   e2Crimson
   superfolder GFP
   

   Use

   Tags

   ssra

   Expression

   Bacterial

   Mutation

   Promoter

   ipaH7.8p and rpsM

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTSAR3.3t

   Plasmid

   #210255

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   DsRed
   superfolder GFP
   

   Use

   Tags

   ssra

   Expression

   Bacterial

   Mutation

   Promoter

   ipaH7.8p and rpsM

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TU#1805_CRISPR_unc-73_exon2

   Plasmid

   #82359

   Purpose

   to create unc-73B null allele

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFM034

   Plasmid

   #40137

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (wild-type) (par-2 )
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   Base pairs 6-523 of par-2 were recoded to create…

   Promoter

   pie-1

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

   Plasmid

   #100849

   Purpose

   AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRCaMP1b
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-ArgiNLS-EGFP

   Plasmid

   #220594

   Purpose

   Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   EGFP
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

   Plasmid

   #161574

   Purpose

   Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

   Depositor

   Robert Malenka, Kevin Beier

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-hKCNQ3-P2A-EGFP

   Plasmid

   #209195

   Purpose

   Cre-dependent expression of Kcnq3 and GFP from the same transcript

   Depositor

   Larry Zweifel

   Article

   Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

   Insert

   Kcnq3 (KCNQ3 Human)
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100852

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-mGreenLantern

    Plasmid

    #164468

    Purpose

    Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    EF1a

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

    Plasmid

    #98927

    Purpose

    Cre dependent AAV expression of GFP spaghetti monster myc construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    GFP-smFP-myc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

    Plasmid

    #98928

    Purpose

    Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    Ruby2-smFP-Flag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hSyn1-SIO-stGtACR2-FusionRed

    Plasmid

    #105677

    Purpose

    Expresses a soma-targeted GtACR2 in Cre-expressing neurons

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-mitoGFP-DIO

    Plasmid

    #174112

    Purpose

    Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

    Depositor

    Maarten Kole

    Article

    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

    Has Service

    AAV2

    Insert

    mitoGFP (COX8A Human)
    

    Use

    AAV

    Tags

    Cox8 targeting sequence and GFP

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

    Plasmid

    #161580

    Purpose

    Express Kir2.1 in cells not expressing Cre.

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

    Plasmid

    #205442

    Purpose

    production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

    Depositor

    Jeremy Kay

    Article

    Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

    Insert

    Unc5c (Unc5c Mouse)
    

    Use

    AAV

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    mini-CMV (with MVM small intron)

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

    Plasmid

    #35510

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

    Plasmid

    #111389

    Purpose

    AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    ChETA-mRuby2
    W3SL
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    W3SL is built from a shortened WPRE, an upstream …

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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