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pYTK001_O2Ab

Plasmid

#183083

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Ab

Plasmid

#183084

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mCherry2-mEGFP

Plasmid

#182859

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mEGFP

Expression

Mammalian

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2364-Tier1-IRES

Plasmid

#169562

Purpose

Tier-1 vector carrying an internal ribosome entry site (IRES-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEYFP-(L)-mApple

Plasmid

#182863

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mApple

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mCherry2-(L)-mApple

Plasmid

#182858

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mApple

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEYFP-(L)-mEGFP

Plasmid

#182852

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mEGFP

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_18bp

Plasmid

#174852

Purpose

Express forward pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_18bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_18bp

Plasmid

#174853

Purpose

Express reverse pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_18bp

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_44bp

Plasmid

#174854

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_44bp

Plasmid

#174855

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDYU1H-TED

Plasmid

#154300

Purpose

A expression vector in Dictyostelium discoideum containing the Hygromycin B resistant gene and the TED module for GFP-negative screening

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Type

Empty backbone

Use

Dictyostelium expression

Promoter

actin15

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDYU1B-TED

Plasmid

#154299

Purpose

A expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screening

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Type

Empty backbone

Use

Dictyostelium expression

Promoter

actin15

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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GGE286

Plasmid

#120776

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T3_Tef

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE256

Plasmid

#120772

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Tef

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE082

Plasmid

#120767

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Tef

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE007

Plasmid

#120758

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_PGM)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P3_PGM

Use

Cloning/assembly plasmid

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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GGE008

Plasmid

#120757

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_GAPdh)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P3_GAPdh

Use

Cloning/assembly plasmid

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0519

Plasmid

#128982

Purpose

Part plasmid for loxP-KIURA3-loxP, Type 6 (yeast marker) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

loxP-KIURA3-loxP

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GGE005

Plasmid

#120748

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_GAPdh)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_GAPdh

Use

Cloning/assembly plasmid

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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