We narrowed to 41,868 results for: tro

1. BM00369

   Plasmid

   #155338

   Purpose

   Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJET1.2_Gli3_Anti-Sense

   Plasmid

   #124440

   Purpose

   Plasmid for anti-sense in situ probe in vitro transcription

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Insert

   GLI-Kruppel family member GLI3 (Gli3 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIBN-hTRF2-tagRFP-T

   Plasmid

   #103812

   Purpose

   BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hTRF2: deletion of amino acids 1-42 and 476 compa…

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR1A-HA-Jag1

   Plasmid

   #46052

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Jag1 (Jag1 Rat)
   

   Use

   Entry vector

   Tags

   HA tag

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-plk1-AKAP

   Plasmid

   #132967

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C terminus of full-length Plk1 fused to the peric…

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXs-hs-HRAS

   Plasmid

   #52730

   Purpose

   retroviral expression of human HRAS

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   HRAS (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCIBN-tagBFP-hNCL

   Plasmid

   #103805

   Purpose

   BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIBN-LacI

    Plasmid

    #103814

    Purpose

    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIBN-EYFP-hNCL

    Plasmid

    #103806

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p53 S23A targeting vector

    Plasmid

    #12167

    Depositor

    Tyler Jacks

    Article

    MacPherson et al EMBO J. 2004 Sep 15. 23(18):3689-99.

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Ser23 mutation (S23A) in exon 2 and puromycin gen…

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-bio-myc-mCE 2-210

    Plasmid

    #112715

    Purpose

    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Use

    Tags

    biotinylation signal peptide (Tagwerker et al. 20…

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid

    #241026

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven empty sgRNA
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-bio-myc-mCE 211-597

    Plasmid

    #112716

    Purpose

    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Use

    Tags

    biotinylation signal peptide (Tagwerker et al. 20…

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-210 (triphosphatase domain)

    Promoter

    CMV

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP

    Plasmid

    #202826

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 - two

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

    Plasmid

    #202825

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 - two

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196881

    Purpose

    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mKate2-DrCavin1a in pT3TS-Dest

    Plasmid

    #194294

    Purpose

    In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1a (cavin1a Zebrafish)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKHH030_sgCtrl#2

    Plasmid

    #174143

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge