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tdTurboRFP-Alpha-Actinin-19

Plasmid

#58050

Purpose

Localization: Alpha Actinin, Excitation: 553, Emission: 576

Depositor

Michael Davidson

Insert

Alpha-Actinin (ACTN1 Human)

Tags

tdTurboRFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles
mTagRFP-T2-Alpha-Actinin-19

Plasmid

#58033

Purpose

Localization: Alpha Actinin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pT7-ZoAl-ENT-3xF-ORF

Plasmid

#238484

Purpose

For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Publication

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1103A

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1

Plasmid

#214671

Purpose

Bacterial expression of GST-tagged mouse GTSF1

Depositor

Phillip Zamore

Publication

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1 (Gtsf1 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-Actinin-19

Plasmid

#57346

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pPERPlucFL

Plasmid

#14811

Depositor

Tyler Jacks

Publication

Reczek et al Mol Cancer Res. 2003 Dec. 1(14):1048-57.

Insert

PERP (Perp Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-RB1

Plasmid

#222550

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RB1

Depositor

George Church

Publication

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RB1 (RB1 Human)

Expression

Mammalian

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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LSSmKate2-Actin-C-18

Plasmid

#56209

Purpose

Localization: Actin, Excitation: 460, Emission: 605

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

LSSmKate2

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos3.2-MCPA2-C-18

Plasmid

#57469

Purpose

Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

MCPA2 (Capza2 Mouse)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mTurquoise-Actin-C-18

Plasmid

#55520

Purpose

Localization: Actin Filaments, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mTurquoise

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCW-HA-hRb-WT-T373A-S608A-S612A-puro

Plasmid

#212674

Purpose

Express tagged hRb mutant

Depositor

Yumi Konagaya, Tobias Meyer

Publication

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

T373A/S608A/S612A

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUG-UBA1

Plasmid

#104665

Purpose

Cloning Module

Depositor

Marco Trujillo

Publication

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-R88Q

Plasmid

#116538

Purpose

Lentiviral expression of PIK3CA R88Q

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

R88Q

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mOrange2-Actin-7

Plasmid

#57943

Purpose

Localization: Actin, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Publication

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Actin (ACTB Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-C71,72,105A

Plasmid

#58396

Purpose

Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRb delta CDK + S230

Plasmid

#58907

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

Depositor

Steven Dowdy

Publication

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S230 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Publication

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221-LETMD1

Plasmid

#132294

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

LETMD1 (LETMD1 Human)

Expression

Mammalian

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR223_PIK3CA_p.H1047Y

Plasmid

#81701

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CA (PIK3CA Human)

Use

Gateway Cloning

Mutation

H1047Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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