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We narrowed to 14,352 results for: Cas9

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Showing: 2461 - 2480 of 14352 results

  1. dCas9-FOG1[N+C]

    Plasmid
    #100085
    Purpose
    dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FOG1(aa 1-45) (ZFPM1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-FOG1-dCas9-FOG1-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100085-plasmid-map-sequence-id-197489

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  2. pMA-SpCas9-g1

    Plasmid
    #80784
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80784-plasmid-map-sequence-id-162402

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  3. pAC2-dual-dCas9VP48-sgExpression

    Plasmid
    #48236
    Purpose
    Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9VP48
    Use
    CRISPR
    Tags
    HA-tag and VP48
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48236-plasmid-map-sequence-id-69543

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  4. SID4x-dCas9-KRAB

    Plasmid
    #106399
    Purpose
    Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain
    Depositor
    Jason Gertz
    Publication
    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SID4x (MXD1 Human)
    KRAB (ZNF10 Human)
    Tags
    FLAG, HA, and NLS
    Expression
    Mammalian
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106399-plasmid-map-sequence-id-207594

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  11. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  13. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

    Plasmid
    #121834
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    x-Cas9(3.7) (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121834-plasmid-map-sequence-id-237388

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  14. pMA-SpCas9-g2

    Plasmid
    #80785
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80785-plasmid-map-sequence-id-162403

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  15. pDIV1049

    Plasmid
    #247970
    Purpose
    CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target mCitrine. pyrG marker
    Depositor
    Uffe Mortensen
    Insert
    Cas9-AMA1-mCit-gRNA-pyrG-Ori-AmpR
    Use
    CRISPR
    Mutation
    none
    Available since
    March 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    247970-plasmid-map-sequence-id-503025

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  16. pCMV-PE2-P2A-hMLH1dn

    Plasmid
    #174827
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-hMLH1dn
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174827-plasmid-map-sequence-id-343635

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  17. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100087-plasmid-map-sequence-id-197556

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  19. pLCKO2::TKOv3

    Pooled library
    #125517
    Purpose
    Human CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backbone
    Depositor
    Jason Moffat
    Publication
    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48227-plasmid-map-sequence-id-69517

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Showing: 2461 - 2480 of 14352 results