We narrowed to 22,436 results for: Sele

1. P2RY11-DuET

   Plasmid

   #213364

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   P2RY11 (P2RY11 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas12b(RuvC)

   Plasmid

   #186450

   Purpose

   Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   E848A, D977A

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-FLAG-APEX2-LGALS8

   Plasmid

   #175758

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS8 (LGALS8 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQTEV-ANP32A

   Plasmid

   #31563

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDuBir-Lbu-dCas13a-avitag

   Plasmid

   #100817

   Purpose

   Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

   Depositor

   Mitchell O'Connell

   Article

   Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

   Insert

   Lbu_Cas13a_R472A_H477A_R1048A_H1053A
   

   Use

   Tags

   AviTag and His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   T7

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. OPN3-DuET

   Plasmid

   #213357

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   OPN3 (OPN3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-scFv-T

   Plasmid

   #67843

   Purpose

   pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

   Depositor

   Joanna Bereta

   Article

   Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

   Insert

   antibody 1D8 light kappa chain
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-Nrxn1α-LNS3

   Plasmid

   #128107

   Purpose

   Express HA tagged Nrxn1α-LNS3

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS3 (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-HA-Nrxn1α-LNS1

   Plasmid

   #128105

   Purpose

   Express HA tagged Nrxn1α-LNS1

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS1 (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 Flag p38 beta2

    Plasmid

    #20355

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 beta2 (MAPK11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQTEV-MAT2B

    Plasmid

    #34795

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    methionine adenosyltransferase II, beta (MAT2B Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRedCmOcr

    Plasmid

    #176226

    Purpose

    A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB and PL

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-mDyn2 pcDNA3

    Plasmid

    #36264

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 2 (Dnm2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pET26b_Nb.AD101

    Plasmid

    #131402

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody AD101
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. hKDR

    Plasmid

    #83436

    Purpose

    human VEGFR2 with V5 and tGFP tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

    Insert

    human VEGFR2 (KDR Human)
    

    Use

    Tags

    V5 and turboGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PMP34-mCherry-EGFP-Tet-On

    Plasmid

    #109017

    Purpose

    Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    PMP34 (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBA-mKeima-PIM

    Plasmid

    #174650

    Purpose

    Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

    Depositor

    Lukas Kapitein

    Article

    Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

    Insert

    xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

    Promoter

    Chicken beta-actin

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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