We narrowed to 18,004 results for: URE

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA

Plasmid

#186410

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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L1-Ck1-hs1-l-chlo

Plasmid

#163937

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region left homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1-Ck4-hs1-r-chlo

Plasmid

#163940

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region right homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1-Ck4-hs2-r-chlo

Plasmid

#163941

Purpose

For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha rbcL_trnR region right homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_MpU6

Plasmid

#136120

Purpose

Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpU6

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS_Cas9-NLS

Plasmid

#136121

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_AtcoCas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12-MpER-Targ

Plasmid

#136094

Purpose

CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

ER targeting peptide from predicted chitinase Mapoly0069s0092

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_eGFP-GHtag-HDEL

Plasmid

#136096

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET30 W96A-GLTP

Plasmid

#170734

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

W96A-GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Mutation

W96A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

Plasmid

#166986

Purpose

Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

Plasmid

#166991

Purpose

Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

Plasmid

#166990

Purpose

Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

Plasmid

#166987

Purpose

Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAWH-IRP

Plasmid

#133896

Purpose

Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

IRP

Expression

Yeast

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-4xUAS

Plasmid

#125149

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB367

Plasmid

#110277

Purpose

gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHEEE_02

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPickles-2.31

Plasmid

#92336

Purpose

FRET-based biosensor for the measurement of BCR-ABL activity

Depositor

Yusuke Ohba

Article

Mizutani et al Clin Cancer Res. 2010 Aug 1;16(15):3964-

Insert

Pickles-2.31

Expression

Mammalian

Promoter

CAG

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-1783-end

Plasmid

#46071

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-1783-end (Map7 Mouse)

Expression

Bacterial

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-856-1351

Plasmid

#46069

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-856-1351 (Map7 Mouse)

Use

Unspecified

Expression

Bacterial

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pAB051e

Plasmid

#79195

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PExt-10cons lambda op-62 gIII, luxAB; Ptet lambda cI-SH2ABL1

Expression

Bacterial

Promoter

PExt-10cons lambda op-62; Ptet

Availability

Academic Institutions and Nonprofits only

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