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pAD-LyseR

Plasmid

#99244

Purpose

For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Publication

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Expression

Bacterial

Promoter

beta-lactamase promoter

Available since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMH-HA

Plasmid

#101767

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

Depositor

Michael Huen

Publication

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.2 TDP-43 5F-L YFP

Plasmid

#84914

Purpose

Mammalian expresion of TDP-43 5F-L YFP

Depositor

Aaron Gitler

Publication

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

F147L, F149L, F194L, F229L, F231L

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCW-Flag-CtIP

Plasmid

#71108

Purpose

mammalian expession of Flag-CtlP

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

TRE promoter, Tet ON

Available since

Nov. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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MP1

Plasmid

#69627

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Publication

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 umuD' umuC recA730

Expression

Bacterial

Promoter

E. coli PBAD

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 actin-3XNLS P2A mCherry

Plasmid

#58475

Purpose

Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST26-hOPTN

Plasmid

#23050

Depositor

Jon Ashwell

Publication

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Optineurin (OPTN Human)

Tags

His

Expression

Mammalian

Mutation

N/A

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCM1.127

Plasmid

#21384

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 promoter (short, no ATG) in pDONRP4P1R

Use

Gateway Cloning

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDEST17-Cdc34a

Plasmid

#18674

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Cdc34A (CDC34 Human)

Tags

His

Expression

Bacterial

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGRG37

Plasmid

#16667

Depositor

Nancy Craig

Publication

McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

Insert

mTn7::Gateway cassette

Expression

Bacterial

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pQLinkGD

Plasmid

#13673

Depositor

Konrad Buessow

Publication

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

Gateway cassette

Use

Co-expression

Tags

GST

Expression

Bacterial

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-GRAB-rDA3m

Plasmid

#208702

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SQSTM1-WT

Plasmid

#194582

Purpose

Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

Depositor

Denes Hnisz

Publication

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SQSTM1 (SQSTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKL2299

Plasmid

#186332

Purpose

This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

Depositor

Kan Wang

Publication

Kang et al Front Plant Sci. 2022 May 4;13:860971. d

Insert

virulence genes from Agrobacterium tumefaciens pTiBo542

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Publication

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

Plasmid

#154983

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-synapsin-HaloCaMP1a-EGFP

Plasmid

#138327

Purpose

chemigenetic calcium indicator

Depositor

Eric Schreiter

Publication

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Has service

AAV1 and AAV9

Insert

HaloCaMP1a

Use

AAV

Tags

EGFP, His-tag, and Nuclear Exclusion Signal

Expression

Mammalian

Promoter

synapsin

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLGB31

Plasmid

#126621

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Publication

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-BRD4ΔN-mCherry-sspB

Plasmid

#121968

Purpose

Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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psgRNA

Plasmid

#114005

Purpose

express sgRNA. ColE1, Kan

Depositor

David Bikard

Publication

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Promoter

BBa_J23119

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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