We narrowed to 59,421 results for: cloning

1. mEmerald-MyosinIC-N-18

   Plasmid

   #62761

   Purpose

   Localization: Cytoskeleton, N1 Cloning Vector, Excitation:487, Emission:509

   Depositor

   Michael Davidson

   Insert

   MyosinIC
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. mEos3.2-MYR-N-5

   Plasmid

   #62769

   Purpose

   Localization: Cytoskeleton , N1 Cloning Vector, Excitation:507 / 572, Emission:516 / 580

   Depositor

   Michael Davidson

   Insert

   MYR
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pBsd-P2a-Fkbp-3xF-GGSG

   Plasmid

   #235514

   Purpose

   N-term tagging construct: clone into ZraI and AfeI sites

   Depositor

   Brian Hendrich

   Insert

   FKBP
   

   Use

   Mouse Targeting

   Tags

   Bsd, FKBP, 3xFLAG

   Mutation

   None

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGSG-FKBP-2HA-P2a-Pac

   Plasmid

   #235521

   Purpose

   C-term tagging construct: clone into PmlI and AfeI sites

   Depositor

   Brian Hendrich

   Insert

   FKBP
   

   Use

   Mouse Targeting

   Tags

   FKBP, 2xHA, Puro

   Mutation

   None

   Available Since

   May 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBsd-T2a-Fkbp-3xF-GGSG

   Plasmid

   #235515

   Purpose

   N-term tagging construct: clone into ZraI and AfeI sites

   Depositor

   Brian Hendrich

   Insert

   FKBP
   

   Use

   Mouse Targeting

   Tags

   Bsd, FKBP, 3xFLAG

   Mutation

   None

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TOgw-C-mEGFP

   Plasmid

   #223953

   Purpose

   Gateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TOgw-C-mECFP

   Plasmid

   #223950

   Purpose

   Gateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Tags

   mECFP

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT-TOgw-C-mEYFP

   Plasmid

   #223951

   Purpose

   Gateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Tags

   mEYFP

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-TOgw-N-mEGFP

   Plasmid

   #223949

   Purpose

   Gateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. UBCi

    Plasmid

    #25599

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UBCi
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. LbPlant toolbox II

    Plasmid Kit

    #1000000263

    Purpose

    Use the LbPlant Toolbox kits for the rapid construction of expression vectors for Lactiplantibacillus plantarum using Golden Gate cloning.

    Depositor

    Georg Fritz

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

12. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. BS II KS+ loxP-GFP_ZEO_loxP

    Plasmid

    #128064

    Purpose

    has loxp-GFP-Zeo-Loxp cassette

    Depositor

    Oskar Laur

    Insert

    loxp-GFP-Zeo-Loxp
    

    Use

    Cre/Lox

    Mutation

    no

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBR HPV31 9E

    Plasmid

    #221009

    Purpose

    Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

    Depositor

    Alison McBride

    Insert

    Human papillomavirus type 31 genome 9E isolate
    

    Use

    Cloning vector

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEmerald-Talin-C-18

    Plasmid

    #62763

    Purpose

    Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Talin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. piggyBAC-U6-GFP-WPRE

    Plasmid

    #200030

    Purpose

    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Lenti puro EF2 LUC-2A-Dendra2

    Plasmid

    #129427

    Purpose

    expression of LUC and Dendra2 reporters under EF2 promoter

    Depositor

    Oskar Laur

    Insert

    LUC Dendra2
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC119-MCS

    Plasmid

    #58807

    Purpose

    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.

    Depositor

    Jen Sheen

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. MZB174

    Plasmid

    #228487

    Purpose

    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    10xHis-SUMO(Smt3)-GST-HRV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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