We narrowed to 10,670 results for: nar

1. LLP618_αGCN4-KRAB-CO

   Plasmid

   #211782

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-mCherry
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect mCherr…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. DEL-pHG165a

   Plasmid

   #90064

   Purpose

   LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   DEL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

   Plasmid

   #120555

   Purpose

   Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   Cas9, NES, ERT2 and GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

   Plasmid

   #199252

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity eSpCas9(1.1)-D10A nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-spCas9-N57

   Plasmid

   #169920

   Purpose

   Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-N57
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N57 is the PAI domain of SB100X

   Promoter

   CAG

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCBE4-gam

   Plasmid

   #124449

   Purpose

   Expresses dead Cas9 (dCas9)-CBE4-gam

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dCBE4-gam
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hnRNPA2_RRM2

   Plasmid

   #157866

   Purpose

   express His tagged hnRNPA2 RRM2

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP784_SnoopCatcher-DNMT3A-CO

   Plasmid

   #211777

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Spy-EZH2
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. hnRNPA2_RRM1

   Plasmid

   #157865

   Purpose

   express His tagged hnRNPA2 RRM1

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFBP-2_6.sensor

    Plasmid

    #162800

    Purpose

    Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    2_6_RNA-device
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2 EGFP sgRNA1

    Plasmid

    #164932

    Purpose

    Expresses EGFP sgRNA1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PVT1-luciferase

    Plasmid

    #113350

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP780_SpyCatcher-KRAB-CO

    Plasmid

    #211773

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-KRAB
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry Codon 59 sgRNA

    Plasmid

    #109431

    Purpose

    MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry codon 59 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRESneo-Ago2

    Plasmid

    #215841

    Purpose

    Expression of AGO2

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

    Insert

    Human AGO2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKD077

    Plasmid

    #136473

    Purpose

    SSB-mTur2 IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-mTur2 (ssb E. coli)
    

    Use

    Tags

    mTurquoise2

    Expression

    Bacterial

    Mutation

    mTurquoise2 inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ires-eGFP(+HAs) donor-1

    Plasmid

    #87865

    Purpose

    Express eGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PVT1-Tol2

    Plasmid

    #113381

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-UbC-mEmerald Rab7a

    Plasmid

    #115238

    Purpose

    To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab7a fused to mEmerald (RAB7A Human)
    

    Use

    AAV

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    7 Amino Acids (21 Nucleotides) between mEmerald a…

    Promoter

    Human UbC

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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