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pRK5-SNAP-CLIP-GB1a

Plasmid

#125709

Purpose

Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Publication

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

SNAP-CLIP-GB1a

Expression

Mammalian

Promoter

CMV

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-SpCas9-BlastR

Plasmid

#107190

Purpose

Confers constitutive expression of SpCas9

Depositor

Richard Sherwood

Publication

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

SpCas9 (NEWENTRY )

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial and Mammalian

Promoter

Chicken ß-actin

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCitrine

Plasmid

#34950

Depositor

Amro Hamdoun

Publication

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CBP-HAT-pET

Plasmid

#21091

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Tags

His and T7 tag

Expression

Bacterial

Mutation

HAT domain aa 1171-1621

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
SUMO_TwinStrep_Cas7-11_crRNA

Plasmid

#197612

Purpose

For protein purification of Cas7-11

Depositor

Feng Zhang

Publication

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Cas7-11 with crRNA

Expression

Bacterial

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7

Plasmid

#170141

Purpose

Expresses CHD7 in insect cells

Depositor

Robert Kingston

Publication

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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[pSK729] pRK5 HA-FLCN(R164A)

Plasmid

#136147

Purpose

mammalian expression of FLCN

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Cell. 2019 Nov 27;179(6):1319-1329.e8. d

Insert

FLCN (FLCN Human)

Tags

HA

Expression

Mammalian

Mutation

R164A

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-GB2

Plasmid

#125710

Purpose

Eukaryotic expression of the GB2 subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Publication

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

GB2

Expression

Mammalian

Promoter

CMV

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Publication

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179119

Purpose

Expresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Publication

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Ezr sgRNA

Use

AAV

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCFP

Plasmid

#28011

Depositor

Lingchong You

Publication

Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

Insert

CFP

Expression

Bacterial

Available since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pNO41

Plasmid

#101067

Purpose

BphP1-PpsR2 chromophore plasmid; ho1

Depositor

Jeffrey Tabor

Publication

Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

Insert

ho1

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-RPA43

Plasmid

#17659

Depositor

Tom Misteli

Publication

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA43 (Twistnb Mouse)

Tags

EGFP

Expression

Mammalian

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GFP-p58

Plasmid

#166942

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

Depositor

Jennifer Lippincott-Schwartz

Publication

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Insert

p58 (Lman1 Rat)

Tags

GFP and chicken lysozyme signal peptide

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPro18-cm

Plasmid

#17807

Depositor

Jay Keasling

Publication

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
BL21ΔACF

Bacterial strain

#102268

Purpose

ompA, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Publication

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial resistance

None

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only
pYES-mtBFP

Plasmid

#45055

Depositor

Benedikt Westermann

Publication

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtBFP

Expression

Yeast

Promoter

GAL

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-pAce-Kv2.1PR

Plasmid

#195523

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

Synapsin

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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