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Plasmid#145576PurposeBacterial Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046826
Plasmid#145687PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP18-B0033-MV_RN
Plasmid#127016PurposeBasic Part RN- RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0033m-MV_RN
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-RBS-pET-MV_RN
Plasmid#127014PurposeBasic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart RBS-pET-MV_RN
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG587
Plasmid#105431PurposeSynthetic biologyDepositorInsertbeta-glucuronidase (2 introns; no ATG-Start codon)
UseSynthetic BiologyMutationBsaI/ BpiI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL 802
Plasmid#52991PurposepLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfpDepositorInsertsOxyS sRNA
ompC
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationcontains −81 to +36 bp relative to start codonPromoterpLlacO-1 and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1148
Plasmid#53006PurposepCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsOxyS sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1070
Plasmid#53002PurposepCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsDsrA sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1057
Plasmid#53001PurposepCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsRhyB sRNA
rpoS
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontainsn −150 to +30 bp relative to start codonPromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-HA-shift-IRES-GFP(KaganE21)
Plasmid#52004PurposeExpresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP markerDepositorInsertMAVS-Shift
UseRetroviralTagsHAExpressionMammalianMutationA 2 nucleotide insertion was introduced between t…Available SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
ExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046757
Plasmid#145657PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046799
Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)/AUG-nLuc-3XFLAG
Plasmid#127299PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP
Plasmid#31481DepositorInsertTraffic Light Reporter 1.1 (Sce target) EF BFP
UseLentiviralMutationmCherry contains M9S and M16L to reduce backgroun…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
Tags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12
Plasmid#221182PurposecdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)DepositorInsertscdGreen
mScarlet-I
ExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen)Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic E2
Plasmid#48747PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtPT4Pro
Plasmid#159414PurposeMedicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtPT4 Promoter
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only