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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Dog 1-77)

Plasmid

#62060

Purpose

Expression of Dog p53 (residues 1-77) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-77

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-gib-FFA

Plasmid

#20043

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

Y489F

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pWZ414-F14

Plasmid

#23063

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 deleted amino acids 3-29

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-gib-FFS

Plasmid

#20039

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

Y489F, A492S

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-gib-LFS

Plasmid

#20041

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

F394L, Y489F, A492S

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-gib-LYS

Plasmid

#20042

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

F394L, A492S

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-gib-LYA

Plasmid

#20044

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

F394L

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pJP_Ctrl04

Plasmid

#230983

Purpose

pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate sfGFP expression.

Depositor

Yolanda Schaerli

Article

Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

Insert

pBAD promoter, no araC

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mNeonGreen

Plasmid

#231288

Purpose

For transiently expressing fusion proteins with N terminally tagged mNeonGreen in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-Renilla

Plasmid

#231291

Purpose

For transiently expressing fusion proteins with N terminally tagged Renilla luciferase in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Renilla

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-StrepIII

Plasmid

#231292

Purpose

For transiently expressing fusion proteins with N terminal StrepIII tags in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mNeonGreen

Plasmid

#231296

Purpose

For transiently expressing fusion proteins with C terminally tagged mNeonGreen in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-Renilla

Plasmid

#231299

Purpose

For transiently expressing fusion proteins with C terminally tagged Renilla luciferase in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Renilla

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-StrepIII

Plasmid

#231300

Purpose

For transiently expressing fusion proteins with C terminal StrepIII tags in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-Cas13bt1-non-targeting (pJK2)

Plasmid

#229054

Purpose

Cloning backbone for Cas13bt1 crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-Cas13bt1-non-targeting (pEZ25)

Plasmid

#229055

Purpose

Cloning backbone for Cas13bt1 crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-RanCas13b-non-targeting (pJK6)

Plasmid

#229056

Purpose

Cloning backbone for RanCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-RanCas13b-non-targeting (pEZ45)

Plasmid

#229057

Purpose

Cloning backbone for RanCas13b crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pUdOm2.2

Plasmid

#210279

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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