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pGEX4T-1_WT_OCT4

Plasmid

#40633

Depositor

James Thomson

Publication

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Promoter

tac

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CYPHR

Plasmid

#14864

Depositor

Alexandra Newton

Publication

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cyan/yellow PH domain reporter (Plcd1 )

Tags

CFP and YFP

Expression

Mammalian

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSIL-eGFP

Plasmid

#52675

Purpose

shRNA silencing plasmid with eGFP transfection marker

Depositor

Johannes Hell

Publication

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Type

Empty backbone

Use

RNAi

Tags

CMV-EGFP

Promoter

CMV and U6

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Publication

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Tags

flag

Expression

Mammalian

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Publication

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Tags

sfGFP

Expression

Mammalian

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ141A

Plasmid

#69290

Purpose

Gateway entry vector; single gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV FLIP FF

Plasmid

#19744

Depositor

Richard Hynes

Publication

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-siRNA control

Plasmid

#10900

Depositor

Bob Weinberg

Publication

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siGFP

Expression

Mammalian

Available since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pETM6

Plasmid

#49795

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Publication

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-Syn-Chronos-tdTomato (AAV8)

Viral prep

#62726-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-Syn-Chronos-tdTomato (#62726). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-tdTomato plasmid DNA. Syn-driven Chronos-tdTomato expression for optogenetic neuronal activation. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Promoter

Syn

Tags

tdTomato

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only
pQE30-His-ThrRS

Plasmid

#124119

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Threonyl-tRNA ligase (thrS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Publication

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pJG/ALPHA MHC

Plasmid

#55594

Purpose

transgene expression driven by ALPHA MHC promoter

Depositor

Jeffrey Robbins

Publication

Gulick et al J Biol Chem. 1991 May 15;266(14):9180-5.

Type

Empty backbone

Expression

Mammalian

Promoter

alpha MHC

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV5)

Viral prep

#198511-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Publication

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Promoter

Syn

Tags

mScarlet (Cre-dependent)

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only
pSUPER.PKCzeta.RNAi

Plasmid

#10803

Depositor

Alex Toker

Publication

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC zeta RNAi (PRKCZ Human)

Use

RNAi

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pPS2991

Plasmid

#8931

Depositor

Pamela Silver

Publication

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NDR2 (STK38L Human)

Tags

FLAG

Expression

Mammalian

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
THBS1-bio-His

Plasmid

#53417

Purpose

Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

THBS1 (THBS1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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μ2-HA-D176A

Plasmid

#32754

Depositor

Alexander Sorkin

Publication

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Tags

HA

Expression

Mammalian

Mutation

D176A

Promoter

CMV

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCMV-L26-Flag

Plasmid

#19972

Depositor

Moshe Oren

Publication

Ofir-Rosenfeld et al Mol Cell. 2008 Oct 24. 32(2):180-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpl26 (RPL26 Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article