We narrowed to 8,708 results for: sgRNA

1. pUC57-Amp_CRISPIE donor B4

   Plasmid

   #172845

   Purpose

   CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mNeonGreen
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-LyzC-GFP-U6ac-ctrl-guides

   Plasmid

   #168240

   Purpose

   "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   control sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pgRNA_hMECP2_promoter_No3

   Plasmid

   #194898

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pgRNA_hMECP2_promoter_No9

   Plasmid

   #194903

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-dSpCas9

   Plasmid

   #92113

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_003 Intergenic control guide 2

   Plasmid

   #193585

   Purpose

   sgRNA control; induces CAS9 cutting in an intergenic region

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgRNA control
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CRISPR sgSHMT2_1

    Plasmid

    #106308

    Purpose

    Express Cas9 and sgRNA targeting SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT-9208

    Plasmid

    #124221

    Purpose

    Cloning plasmid for a self-targeting gRNA library

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HeFm2SpCas9

    Plasmid

    #92111

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    “Highly enhanced Fidelity” mut2 SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLiP-G1

    Plasmid

    #188963

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: atcggtcgcattgttttccactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CRISPR sgSHMT1_1

    Plasmid

    #106311

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIV494

    Plasmid

    #177701

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

    Plasmid

    #173203

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPQDKN0761

    Plasmid

    #185630

    Purpose

    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ141-ZmUbi-RZ-Aa3.8

    Plasmid

    #136376

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pgRNA_hMECP2_promoter_No4

    Plasmid

    #194899

    Purpose

    Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Insert

    sgRNA targeting human MECP2 promoter No.4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti CRISPR V2 sgMTFMT_1

    Plasmid

    #106318

    Purpose

    Express Cas9 and sgRNA targeting MTFMT

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTFMT
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge