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pET21a - TDR

Plasmid

#89627

Purpose

Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

thiol dependent reductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBP-J23108

Plasmid

#72964

Purpose

Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23108 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-J23114

Plasmid

#72965

Purpose

Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23114 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-T7_RBS

Plasmid

#72978

Purpose

T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7+RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTU2-A-lacZ

Plasmid

#72950

Purpose

Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - A site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-B-lacZ

Plasmid

#72951

Purpose

Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - B site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-C-lacZ

Plasmid

#72952

Purpose

Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - C site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-D-lacZ

Plasmid

#72953

Purpose

Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - D site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-B-RFP

Plasmid

#72955

Purpose

Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - B site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-C-RFP

Plasmid

#72956

Purpose

Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - C site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-D-RFP

Plasmid

#72957

Purpose

Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - D site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-b-RFP

Plasmid

#72959

Purpose

Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - b site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
CAG-mCherry-RORbshRNA2

Plasmid

#73984

Purpose

RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse RORb gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-RORbshRNA1

Plasmid

#73985

Purpose

RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse RORb gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-RORbshRNA2

Plasmid

#73986

Purpose

RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse RORb gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-mCherry-RORbshRNA1

Plasmid

#73983

Purpose

RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse RORb gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-mCherry-Otx2shRNA2

Plasmid

#73980

Purpose

Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 ZFYVE9

Plasmid

#67134

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ZFYVE9 (ZFYVE9 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

F1359I

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCGS652

Plasmid

#62220

Purpose

Mammalian expression TALEN vector with N152, C+18 truncations

Depositor

Daniel Voytas

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Type

Empty backbone

Use

TALEN

Tags

AcV5 and SV40 NLS

Expression

Mammalian

Promoter

miniCaggs

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET:CACT:GFP

Plasmid

#60737

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Myc-CICf_AEA

Plasmid

#48188

Purpose

Expresses AEA mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pET-UNC60B

Plasmid

#48740

Purpose

Bacterial expression of UNC-60B (a C. elegans ADF/cofilin)

Depositor

Shoichiro Ono

Article

Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

Insert

C. elegans UNC-60B cDNA (unc-60 Nematode)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET-UNC60A

Plasmid

#48739

Purpose

Bacterial expression of UNC-60A (a C. elegans ADF/cofilin)

Depositor

Shoichiro Ono

Article

Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

Insert

C. elegans UNC-60A cDNA (unc-60 Nematode)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Phb

Plasmid

#48162

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Phb (Phb Mouse)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Slc25a15 mod

Plasmid

#48161

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Slc25a15

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Mrpl1

Plasmid

#48166

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MRPL11 (Mrpl11 Mouse)

Use

Luciferase

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Slc25a15

Plasmid

#48160

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Slc25a15

Use

Luciferase

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 M2 (Y115A, Y148A, Y251A)

Plasmid

#44030

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A Y148A Y251A

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
pFM110

Plasmid

#40156

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SE (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

7SE; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM113

Plasmid

#40159

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N (par-3, par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa1-364)

Promoter

tac

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM107

Plasmid

#40153

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SA (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

7SA; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pFM103

Plasmid

#40149

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, wild-type (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

tac

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM109

Plasmid

#40155

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, C56S (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM108

Plasmid

#40154

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, S241A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM106

Plasmid

#40152

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R163A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM105

Plasmid

#40151

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, K162A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM104

Plasmid

#40150

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R183-5A (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded …

Promoter

T7

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pCR8-DJ1-E64D

Plasmid

#29356

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Mutation

Glu64Asp

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-SpEAF pBacPAK8

Plasmid

#15319

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

ELL associated factor (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
Syn3D71

Plasmid

#14680

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 1-71 …

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Showing: 5001 - 5040 of 9867 results

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