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We narrowed to 7,945 results for: lif

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Showing: 5001 - 5040 of 7945 results

  1. ADEPT-pTarget-RFP

    Plasmid
    #238040
    Purpose
    Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    mRFP1
    Use
    Synthetic Biology
    Promoter
    Lux
    Available Since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238040-plasmid-map-sequence-id-476070

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  3. ADEPT-pTarget

    Plasmid
    #238033
    Purpose
    Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    ssrA
    Promoter
    J23119 (constitutive)
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    238033-plasmid-map-sequence-id-471747

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  8. AAV-hSyn-IvfChr-citrine-KV2.1

    Plasmid
    #221615
    Purpose
    A recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement and soma targeting with KV2.1 motif.
    Depositor
    John Lin
    Article
    Mermet-Joret et al eLife 2023 12:RP90327
    Insert
    vfChrimson-citrine with membrane trafficking enhancement and soma targeting
    Use
    AAV
    Mutation
    vfChrimson-citrine with membrane trafficking enha…
    Promoter
    Human Synapsin
    Available Since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221615-plasmid-map-sequence-id-442201

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  9. pET28Man

    Plasmid
    #203399
    Purpose
    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes
    Depositor
    David Vocadlo
    Article
    Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839
    Insert
    man2A
    Tags
    H6
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    203399-plasmid-map-sequence-id-404714

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  11. pITEdv1

    Plasmid
    #218283
    Purpose
    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218283-plasmid-map-sequence-id-433685

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  12. pITEu2

    Plasmid
    #218282
    Purpose
    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218282-plasmid-map-sequence-id-433684

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  13. pJE13HD7

    Plasmid
    #218281
    Purpose
    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218281-plasmid-map-sequence-id-433683

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  14. pSSA-DsRed/eGFP (pXK-442)

    Plasmid
    #208827
    Purpose
    The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208827-plasmid-map-sequence-id-417251

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  15. pSSA-eGFP (pXK-221)

    Plasmid
    #208826
    Purpose
    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208826-plasmid-map-sequence-id-417242

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  19. pOPO812

    Plasmid
    #195306
    Purpose
    pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5
    Expression
    Bacterial
    Promoter
    Arabinose inducible arapBAD
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195306-plasmid-map-sequence-id-383143

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  20. pOPO646

    Plasmid
    #195307
    Purpose
    pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    TnsD of McCAST
    Expression
    Bacterial
    Promoter
    Arabinose inducible arapBAD
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195307-plasmid-map-sequence-id-383139

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  26. pSCL.040

    Plasmid
    #184974
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184974-plasmid-map-sequence-id-376705

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  28. pSCL.109

    Plasmid
    #184978
    Purpose
    Test effect of extending a1/a2 on LYP1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    LYP1 donor E27stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184978-plasmid-map-sequence-id-376679

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  29. pSCL.107

    Plasmid
    #184976
    Purpose
    Test effect of extending a1/a2 on CAN1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    CAN1 donor G444stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184976-plasmid-map-sequence-id-376676

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  31. pSCL.111

    Plasmid
    #184980
    Purpose
    Test effect of extending a1/a2 on TRP2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    TRP2 donor E64stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184980-plasmid-map-sequence-id-376669

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  34. pSCL.113

    Plasmid
    #184982
    Purpose
    Test effect of extending a1/a2 on FAA1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    FAA1 donor P233stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184982-plasmid-map-sequence-id-376532

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  37. pSCL.039

    Plasmid
    #184973
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184973-plasmid-map-sequence-id-376529

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Showing: 5001 - 5040 of 7945 results