We narrowed to 17,982 results for: ERG

1. pSL0828 (pQCascade_crRNA-4)

   Plasmid

   #130637

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Tag4/hL3MBTL1

   Plasmid

   #28231

   Depositor

   Danny Reinberg

   Article

   Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

   Insert

   l(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Irrelevant S49T mutation

   Available Since

   Feb. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-Tag4/hL3MBTL2

   Plasmid

   #28232

   Depositor

   Danny Reinberg

   Article

   Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

   Insert

   l(3)mbt-like 2 (Drosophila) (L3MBTL2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 22, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

   Plasmid

   #186993

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, non-targeting crRNA guide
   

   Use

   AAV

   Mutation

   s1006-gggs-d1221 deletion

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDM026

   Plasmid

   #216808

   Purpose

   Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Type

   Empty backbone

   Use

   CRISPR; Fungal expression

   Expression

   Bacterial

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

   Plasmid

   #172501

   Purpose

   Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 full locus with Golden gate acceptor site for spacer
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe human FOXC2

   Plasmid

   #15535

   Depositor

   Bob Weinberg

   Article

   Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

   Insert

   FOXC2 (FOXC2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

8. FKBP-CD28TMD-NTEVp(H75E)

   Plasmid

   #137829

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75E)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR_L1-Turbo-NES-YFP-STOP-L2

   Plasmid

   #127358

   Purpose

   gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

    Plasmid

    #186987

    Purpose

    AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-s1006-gggs-d122, Gluc crRNA guide
    

    Use

    AAV

    Mutation

    s1006-gggs-d1221 deletion

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL0762 (pQCascade_crRNA-nt)

    Plasmid

    #130636

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSF107mT-GATEWAY-3'-LAP tag

    Plasmid

    #67618

    Purpose

    A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-termnal LAP (eGFP-TEV-S)

    Expression

    Mammalian

    Promoter

    CMV and SP6

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. scAAV-Mlc2v-MPRA

    Plasmid

    #182649

    Purpose

    Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    rat Mlc2v

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC011 LwCas13a locus with targeting spacer

    Plasmid

    #91903

    Purpose

    LwCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with targeting spacer
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEB2_mScarletI_mVenusNB-MrkHR113A

    Plasmid

    #182292

    Purpose

    expresses c-di-GMP mutant biosensor in E. coli

    Depositor

    Howard Berg

    Article

    Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

    Insert

    mVenusNB-MrkHR113A
    

    Tags

    mVenusNB internal fusion to MrkH with a R113A poi…

    Expression

    Bacterial

    Mutation

    changed arginine 113 to alanine, MrkH numbering …

    Promoter

    proC

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJR021

    Plasmid

    #198814

    Purpose

    Variant of yellow fluorescent protein for transient gene expression

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::destabilizedYFP::let-838 3'UTR
    

    Expression

    Worm

    Mutation

    YFP with destabilized signal from mouse ornithine…

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAD16_CAD_12-490_pvp008

    Plasmid

    #124842

    Purpose

    Expresses Aspergillus terreus CAD in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-11, synthetic codon optimiz…

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe puro H-Ras G12V T35S

    Plasmid

    #12274

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V T35S (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The T35S mutat…

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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