We narrowed to 32,594 results for: LIS

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pcDNA3.4 human alpha5 full

Plasmid

#221396

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

Expression

Mammalian

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

Plasmid

#129722

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-3XFlag

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-B-AtAFB2-mCherry

Plasmid

#129718

Purpose

AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK3 S368A

Plasmid

#122680

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Mutation

changed Serine 368 to Alanine

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-SH3(5R)

Plasmid

#112154

Purpose

Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3-5R (NCK1 Human)

Expression

Mammalian

Mutation

only the second SH3 domain is expressed in this c…

Promoter

CMV

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(mito-LD)

Plasmid

#214421

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-3XHA-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-hPXR-LBD

Plasmid

#67769

Purpose

Expression GST-hPXR-LBD in bacteria

Depositor

Sridhar Mani

Article

Li et al J Biol Chem. 2013 May 10;288(19):13655-6

Insert

human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 107 to 434 of human PXR

Promoter

Tac

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I

Plasmid

#129721

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mScarlet-I

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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OA-1067A1

Plasmid

#164847

Purpose

Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-eGFP

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1067K

Plasmid

#164848

Purpose

Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-tdTomato

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

Plasmid

#111389

Purpose

AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

ChETA-mRuby2
W3SL

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

W3SL is built from a shortened WPRE, an upstream …

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pNICKclos2.0

Plasmid

#73228

Purpose

Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sgRNA to xylR

Use

E.coli - clostridium shuttle vector

Mutation

D10A

Promoter

Pj23119 and Pthl

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-mCherry

Plasmid

#129720

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mCherry

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_His6_NR1.

Plasmid

#170457

Purpose

Expresses histidine tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

Hexa-histidin

Expression

Mammalian

Promoter

Synapsin

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-LD)

Plasmid

#214420

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-FRB-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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iRFP682-Smad2

Plasmid

#118943

Purpose

Mammalian expression of human Smad2 protein

Depositor

Won Do Heo

Article

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

iRFP682-Smad2 (SMAD2 Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP1-V5-APEX2

Plasmid

#228524

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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