We narrowed to 11,276 results for: Vars

1. GeNL(Ca2+)_480/pcDNA3

   Plasmid

   #85205

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_480
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1.2-Hygro

   Plasmid

   #162741

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO Elongation factor 1A

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5 MYC-Smurf2 (FF29/30A) WT

   Plasmid

   #24604

   Depositor

   Jeff Wrana

   Article

   Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

   Insert

   Smurf2 (SMURF2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   F29A, F30A

   Promoter

   Available since

   April 30, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET28a-R4-uAb

   Plasmid

   #101801

   Purpose

   Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

   Depositor

   Matthew DeLisa

   Article

   Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

   Insert

   R4-uAb
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQP-2473

   Plasmid

   #138976

   Purpose

   Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKL13-MgtC bb118

   Plasmid

   #158982

   Purpose

   Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   mgtC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23118 (sigma 70)

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ211_WT_hi

   Plasmid

   #128850

   Purpose

   High copy number plasmid for expression of E. coli flagellin from its natural promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX_313-EGFP

   Plasmid

   #158580

   Purpose

   Lentiviral expression of EGFP

   Depositor

   John Doench, David Root

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW194b63

   Plasmid

   #136930

   Purpose

   Directed evolution of SpCas10

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   npuC-gIII(C)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. BRAF - A12G in pENTR

    Plasmid

    #216606

    Purpose

    gateway cloning donor vector containing BRAF ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    A12G

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKSB-611U6-sgRNA3 AGAG AACA

    Plasmid

    #173673

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMS17: pTwinStrep-SUMO-AvTnsD

    Plasmid

    #168150

    Purpose

    AvTnsD expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsD
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUAS-MEYFP

    Plasmid

    #86372

    Purpose

    membrane targeted EYFP expression in zebrafish

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    EYFP
    

    Use

    Zebrafish

    Tags

    GAP43 membrane targeting

    Expression

    Mutation

    Promoter

    UAS

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330A_FokI-1x2

    Plasmid

    #63602

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR macroH2A1.2-C

    Plasmid

    #30512

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6. ():.

    Insert

    macroH2A1.2-C (Macroh2a1 Mouse)
    

    Use

    Gateway - donor vector

    Tags

    Expression

    Mutation

    NO STOP

    Promoter

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXJ40-GFP-iBox-PAK4cat

    Plasmid

    #190179

    Purpose

    expresses GFP-tagged iBox-PAK4cat

    Depositor

    Bianxiao Cui

    Article

    Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

    Insert

    GFP-iBox-PAK4cat
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUPD-Cyc1t (17-21)

    Plasmid

    #101700

    Purpose

    Cyc1t in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUPD-pTEF2 (01-11)

    Plasmid

    #101685

    Purpose

    pTEF2 in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pV1435

    Plasmid

    #111439

    Purpose

    Solo vector pV1382 + sgCgADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgCgADE2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge