We narrowed to 2,724 results for: gem
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Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dat - EGFP - Tol2
Plasmid#85418Purposefor making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) geneDepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
HypoxCR
Plasmid#59946PurposeA lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.DepositorInsertsGFP-Pest
mCherry-Geminin
UseLentiviralTagsFLAG and PESTExpressionMammalianPromoterCMV and min CMVAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT_tdTomato
Plasmid#138567PurposeUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJDepositorInserttdTomato
UseUnspecifiedAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Ag43
Plasmid#107743PurposeExpresses Ag43 under the control of an L-arabinose transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpBADAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI SMN1
Plasmid#72286PurposeSMN1 exon 7 splicing cassetteDepositorAvailable SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxPromotervegAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PxylA
Plasmid#55178PurposeXylose-inducible promoterDepositorInsertxylA promoter
UseSynthetic Biology; Bacillus biobrick boxPromoterxylAAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx120-20xMS2-Hyg
Plasmid#210220PurposeIntegrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pP19 (GB0038)
Plasmid#68195PurposeProvides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid partDepositorInsertP19 coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCit-APPAPAPPAP-gs
Plasmid#228091PurposeMammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cellsDepositorInsertmCit, APPAPAPPAP
ExpressionMammalianAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pλPR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
ExpressionBacterialPromoterT7Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
RFP-PPPPPPAAAA-gs
Plasmid#228092PurposeMammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cellsDepositorInsertmCit, PPPPPPAAAA
ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDelila (GB0079)
Plasmid#68200PurposeProvides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid partDepositorInsertDelila coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRosea (GB0026)
Plasmid#68194PurposeProvides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid partDepositorInsertA. majus Rosea coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein promoter-less
Plasmid#118008PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only