We narrowed to 6,485 results for: plasmid dna

1. POC1547

   Plasmid

   #226489

   Purpose

   pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three inserts (POC1539-POC1541) assembled in POC1519 using the site-selective methylation protection approach

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. POC1548

   Plasmid

   #226490

   Purpose

   pMOBK360_VM_N3 assembled plasmid (Kanamycin R) containing two inserts (POC1542-POC1543) assembled in POC1519 using the site-selective methylation protection approach

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. POC1549

   Plasmid

   #226491

   Purpose

   pMOBK360_VR_N4 assembled plasmid (Kanamycin R) containing two inserts (POC1544-POC1545) assembled in POC1520 using the site-selective methylation protection approach

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

   Plasmid

   #229797

   Purpose

   Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

   Plasmid

   #229790

   Purpose

   Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

   Plasmid

   #229800

   Purpose

   Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLP-sfgfp

   Plasmid

   #209994

   Purpose

   Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLPT1

   Plasmid

   #209995

   Purpose

   Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   Terminator (TB1006)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLPS1

   Plasmid

   #209992

   Purpose

   Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   RBS (RB0034)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL0_DE_mTurquoise2

    Plasmid

    #202155

    Purpose

    Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    mTurquoise2 gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. HySp5-227

    Plasmid

    #192996

    Purpose

    Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain

    Depositor

    Brigitte Galliot

    Article

    Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

    Insert

    Hydra Sp5
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    no SP box and DNA binding domain

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HySp5-337

    Plasmid

    #192994

    Purpose

    Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

    Depositor

    Brigitte Galliot

    Article

    Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

    Insert

    Hydra Sp5
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    no DNA binding domain

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCL.103

    Plasmid

    #184988

    Purpose

    Express Cas9-P2A-Eco1RT

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCL.027

    Plasmid

    #184964

    Purpose

    Express -Eco1 RT and ncRNA in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(wt), a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCL.102

    Plasmid

    #184987

    Purpose

    Express -Eco1 RT-P2A-Cas9

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-P2A-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCL.006

    Plasmid

    #184970

    Purpose

    Express -Eco1 RT in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Integrating inducible cassette: Eco1 RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCL.005

    Plasmid

    #184971

    Purpose

    Express Cas9 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Integrating inducible cassette: Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOD_003

    Plasmid

    #155362

    Purpose

    Zeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107300

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107299

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge