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pMuLE ENTR H1 R4-R3

Plasmid

#62105

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1 L1-L4

Plasmid

#62104

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1 L1-R5

Plasmid

#62101

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHJ33

Plasmid

#48362

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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1132 pME-Lin28

Plasmid

#49009

Purpose

full length coding sequence for zebrafish lin28 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

lin-28 homolog A (lin28ab Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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666 pME-Lnx-1

Plasmid

#49006

Purpose

full length coding sequence for zebrafish lnx1 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ligand of numb-protein X 2b (lnx2b Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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CS2-HA-dASUN

Plasmid

#47041

Purpose

Expression of tagged-dASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (asun Fly)

Tags

HA

Expression

Mammalian

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
pTTSTOP2

Plasmid

#41752

Depositor

Jeff Gelles

Article

Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

Insert

kinesin heavy chain (Khc Fly)

Expression

Bacterial

Mutation

residues 1-401

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD42-BtuCEDFB

Plasmid

#237541

Purpose

plasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteins

Depositor

Squire Booker

Article

Lanz et al Biochemistry. 2018 Mar 6;57(9):1475-1490

Inserts

btuB
btuF
btuCED

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBad-EBFP2

Plasmid

#14891

Purpose

Bacterial expression of EBFP2 blue fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Has Service

Cloning Grade DNA

Insert

EBFP2

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 light chain

Plasmid

#175358

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 light chain

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 light chain

Tags

N-terminal secretion signal peptide (MEAPAQLLFLLL…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCK011 p5E exorh

Plasmid

#195949

Purpose

Gateway p5E vector

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh regulatory elements

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FAS-GCaMP6f-WPRE

Plasmid

#216277

Purpose

AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

GCaMP6f

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

human synapsin 1 (hSyn)

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mScarlet

Plasmid

#207961

Purpose

Gateway entry vector with the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Use

Gateway entry vector

Promoter

None

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCK004 ubi:mCerulean_SV40 (#302)

Plasmid

#195978

Purpose

Expression construct

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubi:mCerulean

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230 (LbCpf1)

Plasmid

#86210

Purpose

LbCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR; Gateway compatible lbcpf1 entry clone

Tags

5' and 3' NLS

Expression

Plant

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVHA E2F1

Plasmid

#24225

Purpose

Mammalian expression of human E2F1 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ166

Plasmid

#109328

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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