We narrowed to 18,563 results for: REV

1. pEFEX2-A2aGH

   Plasmid

   #160544

   Purpose

   pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFEX1-A2aGH

   Plasmid

   #160546

   Purpose

   pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFEX2-A2aGH

   Plasmid

   #160547

   Purpose

   pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

   Plasmid

   #106469

   Purpose

   Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(S219,D415,H474A)
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Serine 219, Aspartate 415 and Histidine 4…

   Promoter

   YBR139W

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNK3071

   Plasmid

   #219755

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Expression

   Plant

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Expression

   Plant

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. LCV2 STING KO

   Plasmid

   #217445

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   sgRNA targeting STING (STING1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMT-MjtRNA-GFP(47TAG)

   Plasmid

   #193251

   Purpose

   Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Inserts

   GFP(47TAG)
   MjtRNA
   

   Expression

   Bacterial

   Mutation

   Codon for Tyr47 replaced with amber stop codon TA…

   Promoter

   hsp60 and metU

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416-Gal4-dCas9-VP64

   Plasmid

   #71128

   Purpose

   This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

   Depositor

   Ronald Davis

   Article

   Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

   Insert

   Gal4-dCas9-VP64
   

   Tags

   NLS n-terminal, N terminal Gal4 Activator domain,…

   Expression

   Yeast

   Promoter

   Tef1

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNK845

    Plasmid

    #219746

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal luciferase
    

    Use

    Luciferase and Synthetic Biology

    Mutation

    I3S, N4T, F11L, I63T, T99P, T192S, A199P

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AIMTOR A757

    Plasmid

    #140829

    Purpose

    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

    Depositor

    Anne Bonnieu

    Article

    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

    Insert

    AIMTOR A757 (ULK1 Human)
    

    Tags

    NES Nuclear export signal

    Expression

    Mammalian

    Mutation

    The insert comprises only a small sequence of hum…

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1+ Flag-RAP2A

    Plasmid

    #128099

    Purpose

    Expresses RAP2A in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid

    #112713

    Purpose

    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    BirA* (BirA R118G), HIV Rev nuclear localization …

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA320

    Plasmid

    #215952

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AA315

    Plasmid

    #215951

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AA324

    Plasmid

    #215953

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA083

    Plasmid

    #215934

    Purpose

    Fragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO-bio-myc-cCE

    Plasmid

    #112711

    Purpose

    For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    HIV Rev nuclear localization signal (NES), Stop c…

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV with Tet repressor binding sites

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AIMTOR T757

    Plasmid

    #140828

    Purpose

    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

    Depositor

    Anne Bonnieu

    Article

    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

    Insert

    AIMTOR T757 (ULK1 Human)
    

    Tags

    NES Nuclear export signal

    Expression

    Mammalian

    Mutation

    The insert comprises only a small sequence of hum…

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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