We narrowed to 4,489 results for: TIL;

1. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169591

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression
   

   Expression

   Mammalian

   Promoter

   TetO7-PCMVmin-2

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVH617

   Plasmid

   #169606

   Purpose

   Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4 / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CamKII-DIO-hM4D(Gi)-mCherry

   Plasmid

   #190240

   Purpose

   Expresses iDREADD fused to mCherry flanked by DIO

   Depositor

   Young J Yoon

   Article

   Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   mouse CamKII alpha

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE ENTR U6-miR-30 L1-R5

   Plasmid

   #62113

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   RNAi; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CVS-N2c-jGCaMP8f

   Plasmid

   #172387

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   GCaMP8f
   

   Use

   Neurotropic virus

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CVS-N2c-nl.EGFP-tdTomato

   Plasmid

   #172382

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + tdTomato
   

   Use

   Neurotropic virus

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

   Plasmid

   #107317

   Purpose

   Expresses SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9
   

   Use

   CRISPR

   Tags

   3xHA and 2xNLS and NLS

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_VPR_Puro

   Plasmid

   #167887

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HB901: pMVP (L3-L2) polyA

   Plasmid

   #121746

   Purpose

   pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Polyadenylation signal (polyA)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CLM RP HTR

    Plasmid

    #24430

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A

    Available Since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY-HA1-HA2-neo

    Plasmid

    #109123

    Purpose

    FR cassette provider to introduce FR cassette to a BAC by recombineering

    Depositor

    David Gilbert

    Article

    Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

    Inserts

    HA1
    HA2
    

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-27

    Plasmid

    #172752

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-27
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. IF701: pMVP (L5-L4) APEX2

    Plasmid

    #121725

    Purpose

    pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LC501: pMVP (L3-L2) mCherry + polyA

    Plasmid

    #121765

    Purpose

    pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOP461

    Plasmid

    #85934

    Purpose

    Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 4G7 monoclonal antibody
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. JN607: pMVP/SB-DEST

    Plasmid

    #121860

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-hSyn-Gαi*-BERKY1

    Plasmid

    #158427

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pOCC93

    Plasmid

    #118911

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Tags

    eGFP-HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. IZ401: pMVP/Lenti/Neo-DEST

    Plasmid

    #121850

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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