We narrowed to 23,247 results for: crispr

1. pRPR1_c1gRNA_RPR1t

   Plasmid

   #64379

   Purpose

   encodes c1 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   c1 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRPR1_c5gRNA_RPR1t

   Plasmid

   #64383

   Purpose

   encodes c5 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   c5 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Lenti‐Cas9‐2A‐Blast

   Plasmid

   #73310

   Purpose

   Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-casBCDE(+6)

   Plasmid

   #89728

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a longer versi…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-casBCDE(-6)

   Plasmid

   #89730

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-casBCDE(-12)

   Plasmid

   #89731

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF-casBCDE(-18)

   Plasmid

   #89732

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDF-casBCDE(-3)

   Plasmid

   #89729

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPCN

   Plasmid

   #193726

   Purpose

   Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

   Depositor

   Martin Buck

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid

    #126881

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVID

    Plasmid

    #236635

    Purpose

    CRISPR cloning, gRNA expression, and detection of undesired vector insertion during HDR for Drosophila

    Depositor

    Brian McCabe

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    dU6:3

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRAC10A

    Plasmid

    #193734

    Purpose

    Expresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion

    Depositor

    Martin Buck

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid

    #126882

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORFE1052

    Plasmid

    #112078

    Purpose

    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter

    Depositor

    Christian Chevalier

    Insert

    AtCAS9 D10A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized nCas9 D10A S. pyogenes

    Promoter

    2x35S

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORFE0014

    Plasmid

    #112076

    Purpose

    AtCAS9 nickase D10A module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    AtCAS9 D10A without stop codon
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang, Phil Sharp

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGES201

    Plasmid

    #190197

    Purpose

    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHochTypeIII

    Plasmid

    #214039

    Purpose

    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Cas10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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