We narrowed to 11,276 results for: Vars

1. pActinin-TagRFP675-N1

   Plasmid

   #44278

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

   Insert

   TagRFP675
   

   Use

   Tags

   Actinin

   Expression

   Mammalian

   Mutation

   M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

   Promoter

   CMV

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PV247

   Plasmid

   #132338

   Purpose

   CasA constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   CasA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ291_WT_med

   Plasmid

   #128856

   Purpose

   Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCLX-UBI-Tred

   Plasmid

   #27246

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   TurboRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. MS2_SC2_S_Omicron

   Plasmid

   #180229

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Omicron (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Jan. 28, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. MS2_SC2_S_WT

   Plasmid

   #175044

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pBS7

   Plasmid

   #83774

   Purpose

   Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Venus
   

   Use

   Tags

   Venus

   Expression

   Yeast

   Mutation

   F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PonA-BI-Gl-WT-18xPP7-WT-24xMS2 (Switch)

   Plasmid

   #189793

   Purpose

   Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

   Depositor

   Robert Singer

   Article

   Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

   Insert

   beta-globin (Hbb Human, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ponasterone A inducible

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQP-AR10

    Plasmid

    #138980

    Purpose

    Endogenous RAS regulation using light-controlled intrabody

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SNAP-EF(N)-60nmSAH-GFP(1-10)

    Plasmid

    #61663

    Purpose

    2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    SNAP-EF(N)-60nmSAH-GFP(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-3a-His-MBP-TEV-VC_Cas1_WT

    Plasmid

    #120364

    Purpose

    Expresses wildtype V-C Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VC_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-3a-His-MBP-TEV-VD_Cas1_WT

    Plasmid

    #120365

    Purpose

    Expresses wildtype V-D Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVC-CRISPR1

    Plasmid

    #120366

    Purpose

    Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    CRISPR1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVC-CRISPR

    Plasmid

    #120369

    Purpose

    Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Inserts

    CRISPR1
    CRISPR2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVD_CRISPR

    Plasmid

    #120370

    Purpose

    Plasmid containing shortened version of V-D CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLK25

    Plasmid

    #37255

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 727-1630

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pUAS-MmTFP13xmyc

    Plasmid

    #86371

    Purpose

    membrane targeted Tfp1 expression in zebrafish

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    mTfp1
    

    Use

    Zebrafish

    Tags

    3xMyc and GAP43 membrane targeting

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SNAP-EF(N)-5nmSAH-GFP(1-10)

    Plasmid

    #61659

    Purpose

    5nm SAH scaffold with EF Hand and split GFP attachment sites

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    SNAP-EF(N)-5nmSAH-GFP(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLK67

    Plasmid

    #37251

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-728

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only