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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,010 results for: cycs

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Showing: 2541 - 2560 of 9010 results

  1. pAC-ZETA

    Plasmid
    #53316
    Purpose
    Contains Erwinia herbicola Eho10 genes crtE and crtB, together with the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene at the N terminus. Produces zeta-carotene in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    pds
    Use
    Low copy number bacterial cloning vector
    Promoter
    lazZ
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53316-plasmid-map-sequence-id-83035

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  4. pAC-NEUR

    Plasmid
    #53271
    Purpose
    Contains the Erwinia herbicola (Pantoea agglomerans) Eho10 genes crtE and crtB, and the Rhodobacter capsulatus crtI gene and thereby produces neurosporene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtI
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53271-plasmid-map-sequence-id-82987

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  5. pHJ22

    Plasmid
    #48358
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48358-plasmid-map-sequence-id-70047

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  10. pRS416gT-GalL-Cas9

    Plasmid
    #79903
    Purpose
    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
    Depositor
    Ronald Davis
    Article
    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw
    Inserts
    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GPM1 promoter, GalL, and RPR1 promoter with TetO …
    Available Since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    79903-plasmid-map-sequence-id-157872

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  12. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid
    #133318
    Purpose
    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pasteurianus)
    Use
    Unspecified
    Promoter
    xylose
    Available Since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    133318-plasmid-map-sequence-id-266833

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  16. pPEE17

    Plasmid
    #128361
    Purpose
    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Insert
    Acetamidase (amdS)
    Use
    Unspecified
    Promoter
    TEF1
    Available Since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128361-plasmid-map-sequence-id-267942

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  18. pADH139

    Plasmid
    #90987
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, C, mal LEU2 2 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90987-plasmid-map-sequence-id-180520

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Showing: 2541 - 2560 of 9010 results