We narrowed to 7,296 results for: ef1a

1. SpRY dCas9 VPR

   Plasmid

   #188511

   Purpose

   Expresses FLAG tagged SpRY dCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pONSY-CoH2B:Venus

   Plasmid

   #111877

   Purpose

   This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Capsaspora Histone H2B (CoH2B) fused to Venus
   

   Use

   Capsaspora owczarzaki

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV Tmem53-V5

   Plasmid

   #175142

   Purpose

   Lentiviral expression of mouse Tmem53-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem53 (Tmem53 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDEF3‐mTim1 (no tag)

   Plasmid

   #49206

   Purpose

   expresses mouse Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (Havcr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

5. rAAV-Ef1α-EGFP-WPRE-ID-PolyA

   Plasmid

   #241829

   Purpose

   AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

   Depositor

   Benjamin Arenkiel

   Article

   Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

   Insert

   EGFP-WPRE-ID-PolyA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EFS-MCP-3XBFP-2xNLS

   Plasmid

   #236371

   Purpose

   Overexpression of MCP-3XBFP-2xNLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   MCP-3XBFP-2xnls
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-EFS-Halo-NLS-tDeg

   Plasmid

   #236370

   Purpose

   Overexpression of Halo-NLS-tDeg in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   Halo-NLS-tDeg
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

   Plasmid

   #238185

   Purpose

   Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   KLF4 (KLF4 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

   Plasmid

   #238181

   Purpose

   Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   KLF5 (KLF5 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

    Plasmid

    #238184

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 (KLF5 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

    Plasmid

    #238182

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF.DEST51-PTPN12(D199A,C231S)-Clover

    Plasmid

    #215528

    Purpose

    Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    PTPN12 (PTPN12 Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    D199A, C231S (Phosphatase inactive)

    Promoter

    EF1a

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pEF.DEST51-Dok1-Clover

    Plasmid

    #215456

    Purpose

    Expression vector with Dok1 tagged with Clover

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    DOK1 (DOK1 Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pX552-DIO-NLS-mRuby3(with gRNA scaffold)

    Plasmid

    #199583

    Purpose

    Expresses NLS-mRuby3 in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    NLS-mRuby3
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1a

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PlmdCas12e VPR

    Plasmid

    #188516

    Purpose

    Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-VPR

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV Pigb-V5

    Plasmid

    #175160

    Purpose

    Lentiviral expression of mouse Pigb-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Pigb (Pigb Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV V5-Tmem209

    Plasmid

    #175154

    Purpose

    Lentiviral expression of V5-tagged mouse Tmem209

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmem209 (Tmem209 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV Zdhhc6-V5

    Plasmid

    #175146

    Purpose

    Lentiviral expression of mouse Zdhhc6-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Zdhhc6 (Zdhhc6 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV Ptdss2-V5

    Plasmid

    #175143

    Purpose

    Lentiviral expression of mouse Ptdss2-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Ptdss2 (Ptdss2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMCh-664

    Plasmid

    #92252

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    EF1A

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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