We narrowed to 150,454 results for: ins

1. pMOD8A-iRGR-r11

   Plasmid

   #74369

   Purpose

   This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r11
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   AHD1

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6607 MSCV-IP N-HAonly PTPN18

   Plasmid

   #35007

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   PTPN18 (PTPN18 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pAB035a

   Plasmid

   #79190

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 gIII, luxAB; Ptet rpoZ-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   Plac62; Ptet

   Available Since

   Dec. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. POM42-mCitrine

   Plasmid

   #69854

   Purpose

   N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCitrine minus start and stop codon
   

   Expression

   Yeast

   Available Since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Desmocollin2_tail-GST

   Plasmid

   #36992

   Depositor

   Kathleen Green

   Article

   Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

   Insert

   Dsc2 (DSC2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Intracellular domain of Dsc2 (nt 2,603–3,158)

   Available Since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

6. HOXA9 (murine) HIS-tag pET

   Plasmid

   #8492

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HoxA9 (Hoxa9 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. POM43-mCitrine

   Plasmid

   #69855

   Purpose

   N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCitrine minus start and stop codon
   

   Expression

   Yeast

   Available Since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW4.21

   Plasmid

   #62875

   Purpose

   Bacterial expression of MBP-MEG-1

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   MBP-MEG-1
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PI(LM)-Rac1(WT)dc1.FLARE

   Plasmid

   #87590

   Purpose

   PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   PBD
   Rac1
   

   Tags

   YPet and dTurq

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS YBX1

    Plasmid

    #19217

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    YBX1 (YBX1 Chicken)
    

    Expression

    Bacterial

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pET15b-At 14-3-3 phi

    Plasmid

    #44939

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 phi (GF14 PHI Mustard Weed)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pAB029f

    Plasmid

    #79220

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ lambda cI-SH2ABL1; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    PlacZ; PlacIq

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAB031a

    Plasmid

    #79221

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ HA4-Zif268; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    PlacZ; PlacIq

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-At 14-3-3 lambda

    Plasmid

    #44942

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 lambda (GRF6 Mustard Weed)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET28-his-3C_Clu3B6p

    Plasmid

    #100093

    Purpose

    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

    Insert

    14-3-3 sigma human (SFN Human)
    

    Tags

    HSPB6 peptide and His6

    Expression

    Bacterial

    Mutation

    75-77 residues changed to AAA to facilitate cryst…

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAB043c

    Plasmid

    #79223

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ lambda cI-p38-alpha; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    PlacZ; PlacIq

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRPB1-CTD8

    Plasmid

    #112820

    Purpose

    pSMF2 with 8 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRPB1-CTD10

    Plasmid

    #112821

    Purpose

    pSMF2 with 10 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB042b

    Plasmid

    #79193

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7
    

    Expression

    Bacterial

    Promoter

    Plac62; Ptet

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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