We narrowed to 6,032 results for: plasmid dna

1. pFETCh_KMT2B

   Plasmid

   #101070

   Purpose

   Donor vector for 3' FLAG tag of human KMT2B

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   KMT2B homology arms (KMT2B Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. sTR060

   Plasmid

   #177370

   Purpose

   Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

   Depositor

   Jerome Bonnet, Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   Trigger RNA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZJQIBEBT003

   Plasmid

   #140053

   Purpose

   The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

   Depositor

   Jia Zhang

   Article

   Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

   Insert

   eMutS-L157C-G233C-CBM-EGFP
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   L157C, G233C

   Promoter

   T7

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV.U6.gLacZ.Cas9-T2A-GFP

   Plasmid

   #170361

   Purpose

   Negative control plasmid used for Crispr/cas9 based disruption.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFETCh_ARID4B

   Plasmid

   #101072

   Purpose

   Donor vector for 3' FLAG tag of human ARID4B

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ARID4B homology arms (ARID4B Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFETCh_RERE

   Plasmid

   #101069

   Purpose

   Donor vector for 3' FLAG tag of human RERE

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   RERE homology arms (RERE Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_RERE_1

   Plasmid

   #101073

   Purpose

   Encodes gRNA for 3' target of human RERE

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against RERE (RERE Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold

   Plasmid

   #226933

   Purpose

   Encoding Nme2ABE8e and sgRNA cassette on a single AAV

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   TadA8e, nNme2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

    Plasmid

    #226935

    Purpose

    Encoding SaKKHABE8e and sgRNA cassette on a single AAV

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    TadA8e, nSaKKHCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA201

    Plasmid

    #216026

    Purpose

    Fragmid fragment: (Cas protein) firefly luciferase

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Fluc_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA156

    Plasmid

    #216022

    Purpose

    Fragmid fragment: (Cas protein) Nanobody binds ALFA tag

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NbALFA_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AA349

    Plasmid

    #216032

    Purpose

    Fragmid fragment: (Cas protein) Renilla luciferase

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Rluc_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-DDK_V222A RAD23B

    Plasmid

    #201580

    Purpose

    Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation V222A

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_F400A RAD23B

    Plasmid

    #201581

    Purpose

    Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation F400A

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL.178

    Plasmid

    #184999

    Purpose

    Express -Eco1 FANCF editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    FANCF donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCL.176

    Plasmid

    #184997

    Purpose

    Express -Eco1 RNF2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    RNF2 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCL.179

    Plasmid

    #185000

    Purpose

    Express -Eco1 HEK4 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK4 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX458_ARID4B_1

    Plasmid

    #101078

    Purpose

    Encodes gRNA for 3' target of human ARID4B

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ARID4B (ARID4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TBXTA-c043

    Plasmid

    #139766

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, WT (TBXT Human)
    

    Use

    Tags

    Avi-tag (Biotin) and His6-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids E41- D225

    Promoter

    T7

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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