We narrowed to 87,726 results for: lias

1. pcDNA3HA-AMOT p130∆PPxY1

   Plasmid

   #166711

   Purpose

   expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 L129A P130A
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed leucine 129 to alanine, proline 130 to al…

   Promoter

   CMV promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mAkt-ER

   Plasmid

   #39530

   Depositor

   Julian Downward

   Article

   Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

   Insert

   Akt (Akt1 Mouse)
   

   Use

   Tags

   ER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA-HA-hTRAF3 88-C

   Plasmid

   #44034

   Depositor

   Shao-Cong Sun

   Article

   Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

   Insert

   TNF receptor-associated factor 3 (TRAF3 Human)
   

   Use

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   Contains amino acids 89-568; V274D and S393G

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pDx_mScarlet-I-SYFP2

   Plasmid

   #189761

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I-SYFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. dTomato_LD 0_CS M_tTA-BFP

   Plasmid

   #58875

   Purpose

   MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_HLA-cmyc-GRM3 C127A

   Plasmid

   #140451

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Akt(R25C)

   Plasmid

   #39532

   Depositor

   Julian Downward

   Article

   Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

   Insert

   Akt(R25C) (Akt1 Mouse)
   

   Use

   Tags

   6G linker, EGFP, and HA

   Expression

   Mammalian

   Mutation

   R25C

   Promoter

   Available since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pRK-flag-USP19 494-1318

   Plasmid

   #78587

   Purpose

   Expression flag-USP19 494-1318 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 494-1318 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-493

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP_E1770Q-talin1 (1-2541)

   Plasmid

   #166121

   Purpose

   For expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   E1770Q-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E1770Q

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL7.0: Venus-oLID-Mito (From ActA)

    Plasmid

    #60414

    Purpose

    Mammalian Expression of Venus-oLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. optoPKAW196R/F327A

    Plasmid

    #104547

    Purpose

    Expresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R/F327A
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R and Y204A

    Promoter

    CMV

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-D2ir promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcdna3 T7 Jip1b

    Plasmid

    #52122

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

14. FnoCas12a-DR-cr in PBCSK-

    Plasmid

    #126642

    Purpose

    Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL7.0: Venus-oLID-CAAX (from KRas4B)

    Plasmid

    #60412

    Purpose

    Mammalian Expression of Venus-oLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mSTIM1_1-310-CFP

    Plasmid

    #69551

    Purpose

    Expresses a STIM1_1-310 fragment mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    mSTIM1_1-310
    

    Use

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5-FRT-TO-EGFP-RIP2_S176A

    Plasmid

    #131202

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

    Insert

    RIPK2 S176A (RIPK2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S176A

    Promoter

    pCMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Ago3m

    Plasmid

    #21531

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago3 (AGO3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    missing aa757-823, the C-termianl 66aa of PIWI do…

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

19. CD4_LD 0_CS G_tTA-BFP

    Plasmid

    #58869

    Purpose

    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI-Flag-NEDD4L∆WW2

    Plasmid

    #166708

    Purpose

    expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L W453A
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 453 to alanine

    Promoter

    CMV promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCI-Flag-NEDD4L∆WW3

    Plasmid

    #166709

    Purpose

    expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L W525A
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 525 to alanine

    Promoter

    CMV promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FnoCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126644

    Purpose

    Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(+) HIPK3 Antisense

    Plasmid

    #60635

    Purpose

    Negative Control for the pcDNA3.1(+) HIPK3 Sense plasmid

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Homeodomain-interacting protein kinase 3 (HIPK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)

    Plasmid

    #69904

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F169L in ZKSCAN1

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK-flag-USP19 494-1290

    Plasmid

    #78580

    Purpose

    Expression flag-USP19 494-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 494-1290 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-493 and mutated 1291 residue…

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3.1-mCherry-CyrCR1.miR17

    Plasmid

    #128748

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-His:FRB:nls:VPR

    Plasmid

    #135987

    Purpose

    Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    FRB domain fused to the VPR activation domain
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry_LD 0_CS M_tTA-BFP

    Plasmid

    #58874

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry_LD 0_CS A_tTA-BFP

    Plasmid

    #58873

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEBB-3XMyc-TRAF2 S274A

    Plasmid

    #44107

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S274A (TRAF2 Human)
    

    Use

    Tags

    3xMyc

    Expression

    Mammalian

    Mutation

    Serine 274 to Alanine

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only