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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,769 results for: 109

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Showing: 2561 - 2580 of 13769 results

  1. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

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  6. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid
    #176087
    Purpose
    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Lys72 to Ala, and mutation in the PAM…
    Promoter
    CMV
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    176087-plasmid-map-sequence-id-352865

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  8. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  10. PF3D7_1350600-COMP-blac-flag-his

    Plasmid
    #110968
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    PF3D7_1350600 (PF3D7_1350600 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110968-plasmid-map-sequence-id-224245

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  11. GPR180-COMP-blac-flag-his

    Plasmid
    #110955
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    PF3D7_1213500 (PF3D7_1213500 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110955-plasmid-map-sequence-id-221974

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  13. Sctrans-PP

    Pooled Library
    #153101
    Purpose
    CRISPR library targets all non-essential and conditionally essential transporter genes in S. cerevisiae
    Depositor
    Irina Borodina
    Article
    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(
    Expression
    Yeast
    Species
    Saccharomyces cerevisiae
    Use
    CRISPR
    Available Since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  20. MAEBL-COMP-blac-flag-his

    Plasmid
    #110963
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    merozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110963-plasmid-map-sequence-id-223595

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Showing: 2561 - 2580 of 13769 results