We narrowed to 8,515 results for: 187

1. pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218766

   Purpose

   encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-VSMA*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y37V, D167G, D182S, F183M, L186A, D265R

   Promoter

   tacI

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218767

   Purpose

   encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-pAAFRS-9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y37G, L71V, D182C, F183Y, L186C, D265R

   Promoter

   tacI

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-IFT38

   Plasmid

   #218723

   Purpose

   Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT38 (CLUAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST_A0A061ACN9_CAEEL (pBS0349)

   Plasmid

   #185187

   Purpose

   For the mammalian expression of the C. elegans protein A0A061ACN9_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   A0A061ACN9_CAEEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS1385 TDP43 RRM-GFP VLIM-S

   Plasmid

   #118792

   Purpose

   To test the effect of sequence on TDP43 RRM-GFP droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All VLIM to S in CTD of TDP43 RRM-GFP reporter

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBS1384 TDP43 RRM-GFP FYW-L

   Plasmid

   #118793

   Purpose

   To test the effect of sequence on TDP43 RRM-GFP droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All FYW to L in CTD of TDP43 RRM-GFP reporter

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti_Neo_ZFP64_N_3XFLAG

   Plasmid

   #118700

   Purpose

   Express 3xflag tagged ZFP64

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Insert

   ZFP64 (ZFP64 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG was fused to the N terminal of ZFP64 cDNA

   Expression

   Mutation

   Promoter

   EF1A

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1 Flag-Sesn2 419A/422A/426A

   Plasmid

   #61872

   Purpose

   Lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant

   Depositor

   Ming Li

   Article

   Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

   Insert

   Sesn2 (Sesn2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   419A/422A/426A

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Cas12aUltra_5XNLS

   Plasmid

   #218775

   Purpose

   His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

   Depositor

   Ross Wilson

   Article

   Sahu et al bioRxiv 2024.07.14.603391

   Insert

   5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
   

   Use

   CRISPR

   Tags

   6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

   Expression

   Bacterial

   Mutation

   Nucleotides mutations were made to encode for arg…

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti HRE-Luc pGK Hygro

    Plasmid

    #118706

    Purpose

    reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    HRE (HIF-responsive promoter (3xHRE)-luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5B-FRT-control-APEX

    Plasmid

    #73187

    Purpose

    Expresses "control"-APEX (cytosolic) in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    control-APEX (Nphp3 Glycine max, Mouse)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    changed glycine 2 to alanine

    Promoter

    EF1alpha

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-GST-His6-2_HBD

    Plasmid

    #171870

    Purpose

    Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Tandem repeats Hybrid binding domain of Rnase H1
    

    Use

    Tags

    GST- and His10-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid

    #218778

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(3)

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti HRE mutant-Luc pGK Hygro

    Plasmid

    #118707

    Purpose

    reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    HREmutant-Luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C1-BBS7

    Plasmid

    #218715

    Purpose

    Expresses N-terminally EGFP-tagged BBS7 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS7 (BBS7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTight-hASCL1-N174

    Plasmid

    #31876

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    achaete-scute complex homolog 1 (ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-shCTRL

    Plasmid

    #181875

    Purpose

    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    control shRNA with no known targets in the mouse genome
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-EGFP-C-IFT52

    Plasmid

    #218725

    Purpose

    Expresses N-terminally EGFP-tagged IFT52 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

    Insert

    IFT52 (IFT52 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-BBS5

    Plasmid

    #218714

    Purpose

    Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS5 (BBS5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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