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pFRT/TO/HIS/FLAG/HA-HNRNPR

Plasmid

#38067

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPR heterogeneous nuclear ribonucleoprotein R (HNRNPR Human)

Tags

FLAG, HA, and His

Expression

Mammalian

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AcKRS

Plasmid

#140021

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AcKRS-3 (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

L301M, L305I, Y306F, L309A, C348F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKOv3

Plasmid

#209046

Purpose

Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA

Plasmid

#108730

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

Plasmid

#108833

Purpose

Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to aa396

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS and GAI

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

Plasmid

#159579

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStk11#2/Cre

Plasmid

#173653

Purpose

Expresses a Stk11-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stk11 (Stk11 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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β₂AR-mCFP

Plasmid

#38260

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

β₂AR (ADRB2 Human)

Tags

Flag, mCFP, and signal peptide sequence (MKTIIALS…

Expression

Mammalian

Mutation

diglycine linker (replacing terminator codon)

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFRT/TO/HIS/FLAG/HA-YTHDF2

Plasmid

#38089

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

YTHDF2 YTH domain family, member 2 (YTHDF2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318)

Plasmid

#204193

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317, 318

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318; A341)

Plasmid

#204194

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA

Plasmid

#108748

Purpose

Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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CaMK2rep

Plasmid

#239622

Purpose

CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

Depositor

F Javier Díez Guerra

Article

Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

Insert

SpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)

Tags

Spot, myc, and sGFP2

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLV EF1alpha INTS4 K26/27A IRES Cherry

Plasmid

#228111

Purpose

Lentiviral expression of human INTS4 K26/27A

Depositor

Tom Misteli

Article

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Mutated Lysine 26 and 27 to Alanine

Promoter

EF 1alpha

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-KIAA1967

Plasmid

#38082

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

KIAA1967 (CCAR2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pYTRW22K_7Ti1

Plasmid

#177293

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

lacZ

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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KHBD00297

Plasmid

#34423

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10798 (Myc Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
WP_010880027.1

Plasmid

#194729

Purpose

Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

Depositor

Fang Li

Article

Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

Insert

nanoparticle-proteinA and His tag

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PHD2

Plasmid

#21401

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN1 (EGLN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pEMS2113

Plasmid

#49138

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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