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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_CS M_tTA-BFP

Plasmid

#58874

Purpose

MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pMT1100

Plasmid

#8618

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa3, barstar

Tags

phoA signal sequence to RNase Sa3

Expression

Bacterial

Mutation

In addition to the substitution of the barnase ge…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
mCherry_LD 0_CS A_tTA-BFP

Plasmid

#58873

Purpose

MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pIW069

Plasmid

#134133

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSPVpr-hp

Plasmid

#48119

Purpose

Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

signal peptide of Vpr

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-zebrafish lyn(C466A)–GFP

Plasmid

#35957

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Tags

EGFP

Expression

Mammalian

Mutation

C466A

Promoter

cmv

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-lyn C466A-GFP

Plasmid

#35038

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

Lyn C466A-GFP (lyn Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97deltaD2(K251A)

Plasmid

#17230

Depositor

Hemmo Meyer

Article

Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

Insert

p97 (Vcp Mouse)

Tags

His

Expression

Bacterial

Mutation

change codon 457 to stop codon, change lysine 25…

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
mEos2-MyosinIIA-C-18

Plasmid

#57403

Purpose

Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pA_higher-CheY-pMag-mNeonGreen

Plasmid

#253260

Purpose

Encodes the fusion of CheY, pMag, and mNeonGreen driven with the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

CheY_pMag_mNeonGreen

Tags

pMag and mNeonGreen

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT3-neo

Plasmid

#250304

Purpose

Encodes gRNA targeting FUT3

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT3 gRNA (FUT3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT5-neo

Plasmid

#250305

Purpose

Encodes gRNA targeting FUT5

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT5 gRNA (FUT5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBS L30 ALFA-Apex-Rab7A

Plasmid

#224892

Purpose

Rab7A fused to APEX and an ALFA tag on N-term in a pBS L30 backbone.

Depositor

Raphael Gaudin

Article

Bare et al bioRxiv 2025.10.07.680694

Insert

Rab7A (RAB7A Human)

Tags

ALFA and APEX2

Expression

Mammalian

Promoter

Gibson Cloning

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti_Pemax_t2a_GFP-Kash

Plasmid

#238541

Purpose

Lentiviral expression of Pemax editor with T2A to GFP KASH

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Insert

Pemax-T2A-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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hSyn1-mCherry-CAAX

Plasmid

#223674

Purpose

AAV expression of mCherry from hSyn1 promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.S29W.A95K.mRuby3

Plasmid

#244911

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mRuby3

Use

AAV

Mutation

S29W.A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95A.A119L.mIRFP670nano3

Plasmid

#244917

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mIRFP670nano3

Use

AAV

Mutation

A95A.A119L

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mIRFP670nano3

Plasmid

#244916

Purpose

iATPSnFR2.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mIRFP670nano3

Use

AAV

Mutation

A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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